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Category
Primary Antibodies
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Long description
Recognizes a ~102kDa identified as MSH2. Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non-polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites / simple repeatative sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurance of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutSbeta, which binds to insertion/deletion loops in DNA.
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Antibody come from
Recombinant protein derived from the extracellular domain of the chicken TGF III receptor protein. Protein shares significant homology with the mouse, rat and human sequences.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
2MSH01
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Antigen antibody binding interaction
Mouse anti Mismatch Repair Protein 2 (MSH2) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry (Frozen & Paraffin Sections)
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Antibody s suited for
Antibody can be used for Western blotting (5-10 µg/ml), immunoprecipitation (5 µg/mg of protein lysate and immunohistochemistry on paraffin embedded tissue sections (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Brown, C.B., et al. 'Requirement of type III TGF- receptor for endocardial cell transformation in the heart.' Science 1999, 283, 2080-2082
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
MSH2-OT1, MSH2
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Short name
Mouse anti Mismatch Repair Protein 2 (MSH2)
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Technique
Mouse, anti, antibody to, mouses
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Host
mouse
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Isotype
IgG1
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Label
unlabeled
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Mismatch Repair Protein 2 (mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli))
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Alternative technique
murine, antibodies
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Alternative to gene target
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli), COCA1 and FCC1 and HNPCC and HNPCC1 and LCFS2, MSH2 and IDBG-50900 and ENSG00000095002 and 4436, ADP binding, nuclei, Msh2 and IDBG-201483 and ENSMUSG00000024151 and 17685, MSH2 and IDBG-633627 and ENSBTAG00000002742 and 533115
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