Autophagy Immunoblot Kit (LC3-II Detection)

Shipping Temp

Blue Ice
Storage Temp

Upon receipt, store Anti-LC3 antibody and Secondary antibody at -20ºC. Store all other components at room temperature.
Detection Method

Immunoblot
Category Product Type

Kit
Datasheet
SDS

more (CBA-5115) GHS.pdf
Description

The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

Gene target

Autophagy Immunoblot Kit LC3-II
Gene symbol

MAP1LC3A, SPINK2, CRABP2, MGST2, IBSP, APOL2, MGAT2, P4HA2, XYLT2, TMED10P1
Short name

Autophagy Immunoblot Kit (LC3-II )
Technique

detection
Alternative name

Autophagy Immunoblot reagent (LC3-II quantification)
Alternative technique

kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:11245
Gene

SPINK2
Long gene name

serine peptidase inhibitor Kazal type 2
Synonyms gene name
- serine protease inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
Synonyms
- HUSI-II
Synonyms name
- acrosin-trypsin inhibitor
GenBank acession
- BC022514
Locus

4q12
Discovery year

1993-12-01
Entrez gene record

6691
Pubmed identfication
- 8428671
RefSeq identity
- NM_021114
Classification
- Serine peptidase inhibitors, Kazal type
VEGA ID

OTTHUMG00000128769

Identity

HGNC:7045
Gene

MGAT2
Long gene name

alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Synonyms gene name
- mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Synonyms
- GNT-II
GenBank acession
- U15128
Locus

14q21.3
Discovery year

1993-02-16
Entrez gene record

4247
Pubmed identfication
- 7635144
RefSeq identity
- NM_002408
Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
VEGA ID

OTTHUMG00000140271
Locus Specific Databases
- Congenital Disorder of Glycosylation pages

Identity

HGNC:8547
Gene

P4HA2
Long gene name

prolyl 4-hydroxylase subunit alpha 2
Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
- prolyl 4-hydroxylase, alpha polypeptide II
Synonyms
- C-P4Halpha(II)
Synonyms name
- 4-PH alpha 2
- collagen prolyl 4-hydroxylase alpha(II)
GenBank acession
- U90441
Locus

5q31.1
Discovery year

1999-02-23
Entrez gene record

8974
Pubmed identfication
- 9211872
- 9149945
RefSeq identity
- NM_004199
Classification
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000059647

Identity

HGNC:30754
Gene

TMED10P1
Long gene name

transmembrane p24 trafficking protein 10 pseudogene 1
Synonyms gene
- TMED10P
Synonyms gene name
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene 1
Synonyms
- Tmp21-II
GenBank acession
- AJ004914
Locus

8q24.3
Discovery year

2005-08-26
Entrez gene record

286102
Pubmed identfication
- 10376215
RefSeq identity
- XR_002807
VEGA ID

OTTHUMG00000165203

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data