EZCatch™ GST-Spin Purification Kit

  • Catalog number
    K861-10
  • Price
    Please ask
  • Size
    10 columns
  • Description
    Contains 10 columns and sufficient reagents for easy & efficient purification of a total of ~2.5 mg of recombinant GST-fusion proteins from 10 different samples.. The expression and solubility of recombinant proteins can be increased by tagging with Glutathione S-transferase (GST). GST-tag binds to glutathione with high affinity and specificity enabling purification of GST-tagged proteins using glutathione-based affinity resins. EZCatch™ GST-Spin Purification Kit is designed for fast, specific and easy purification of such GST-tagged proteins from cell lysates. This kit contains 10 easy-to-use spin columns allowing one-step high quality affinity purification of different GST-tagged proteins. In addition, the kit contains a proprietary Lysis Buffer allowing quick preparation of clear lysates from cell pellets without the need for sonication. Eluted proteins can be directly used for downstream analysis.
  • Product Highlights
    Binding Capacity: Each Spin column can bind upto 250 µg of recombinant protein from 1-5 ml of cell culture or up to 0.25 g of cell pellet. Column Content: Each Spin column contains 100 µl of high performance Glutathione Sepharose beads (Cat # 6555). Bead Content: A 50% slurry (100 µl of settled resin is equivalent to 200 µl of 50% slurry) in 20% ethanol. • Rapid purification of GST-fusion proteins from multiple samples • Optimization of growth conditions for the expression of recombinant proteins • Pull-down of purified GST-fusion proteins for subsequent Western blot (with GST Antibody – Cat # 3997), ELISA and other downstream applications
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Test
    Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
  • Gene target
  • Gene symbol
    MGST3
  • Short name
    EZCatch™ GST-Spin Purification Kit
  • Technique
    Purification, purified
  • Alternative name
    EZCatch™ GST-Spin purification reagent
  • Alternative technique
    purifications, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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