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EZCatch GST Spin Purification Kit

EZCatch GST Spin Purification Kit is available 2 times from Biovision labs

K861-10 | EZCatch GST-Spin Purification Kit size: 10 columns | 387.47 USD

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K861-10 | EZCatch™ GST-Spin Purification Kit size: 10 columns | 286.13 USD

Catalog number K861-10
Supplier Biovision
Price 286.13 USD
Size 10 columns
Gene targetEZCatch™ GST-Spin Kit
Short name EZCatch™ GST-Spin Purification Kit
Alternative name EZCatch™ GST-Spin purification reagent
1. Gene info
Identity 7064
Gene MGST3
Long gene name microsomal glutathione S-transferase 3
Synonyms
  • GST-III
Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
GenBank acession
  • AF026977
Locus 1q24.1
Discovery year 1997-10-16
Entrez gene record 4259
Pubmed identfication
  • 9278457
RefSeq identity
  • NM_004528
Classification
  • Microsomal glutathione S-transferases
Havana BLAST/BLAT OTTHUMG00000034627
Product images
    #0#.jpegEZCatch™ GST-Spin Purification Kit
Taglines Contains 10 columns and sufficient reagents for easy & efficient purification of a total of ~2.5 mg of recombinant GST-fusion proteins from 10 different samples.
Packaging EA
Storage Temp. +4°C
Shipping gel pack
Shelf Life 12 months
Category Protein Purification
Highlights Binding Capacity: Each Spin column can bind upto 250 µg of recombinant protein from 1-5 ml of cell culture or up to 0.25 g of cell pellet. Column Content: Each Spin column contains 100 µl of high performance Glutathione Sepharose beads (Cat # 6555). Bead Content: A 50% slurry (100 µl of settled resin is equivalent to 200 µl of 50% slurry) in 20% ethanol. • Rapid purification of GST-fusion proteins from multiple samples • Optimization of growth conditions for the expression of recombinant proteins • Pull-down of purified GST-fusion proteins for subsequent Western blot (with GST Antibody – Cat # 3997), ELISA and other downstream applications
Description The expression and solubility of recombinant proteins can be increased by tagging with Glutathione S-transferase (GST). GST-tag binds to glutathione with high affinity and specificity enabling purification of GST-tagged proteins using glutathione-based affinity resins. BioVision’s EZCatch™ GST-Spin Purification Kit is designed for fast, specific and easy purification of such GST-tagged proteins from cell lysates. This kit contains 10 easy-to-use spin columns allowing one-step high quality affinity purification of different GST-tagged proteins. In addition, the kit contains a proprietary Lysis Buffer allowing quick preparation of clear lysates from cell pellets without the need for sonication. Eluted proteins can be directly used for downstream analysis.
Storage condition +4°C
Shipping condition gel pack
Maximum time for storage 12 months
Test Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
Technique Purification
Alternative technique purifications
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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EZCatch™ GST-Spin Purification Kit | Technique alternative |
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EU:+32-(0)1-658-90-45 US:+1-(408)780-0908 [email protected]
  • EZCatch
  • GST-Spin
  • Kit
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