This antibody has been succesfully tested for use in following methods: Flow cytometry
Target of this antibody: CD54/ICAM1
Formulation of this antibody: Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
Alternative names of antibody target: CD54/ICAM1
Recommended usage: Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (0.5 µg).
Storage information: Shelf time: 12 months in temp.: 4 deg.C
Shipping conditions: gel pack
Antibody host: Mouse
This antibody reacts with: Human
Clonality of antibody: Monoclonal
Isotype: Mouse IgG1
Purification method: Affinity chromatography.
Immunogene sequence: Human CD54/ICAM1
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: Inter-Cellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds. The 15.2 antibody reacts with the 85-110 kDa ICAM-1.
Form of antibody: Liquid
Concentration of antibody: N/A
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
Conjugation Anti-FITC Antibody
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
French translation anticorps
Short name anti-CD54 (ICAM1) FITC Monoclonal Antibody (Clone 15.2)
Technique Antibody, anti, FITC
Alternative name antibody to-CD54 (intercellular adhesion molecule 1) fluorecein monoclonal (Antibody to) (clonality 15.2)
Alternative technique antibodies
Alternative to gene target intercellular adhesion molecule 1, BB2 and CD54 and P3.58, ICAM1 and IDBG-26804 and ENSG00000090339 and 3383, protein complex binding, Extracellular, Icam1 and IDBG-138668 and ENSMUSG00000037405 and 15894, ICAM1 and IDBG-643247 and ENSBTAG00000010303 and 281839
Clone name 15.2