IFN-λ2, human recombinant
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Catalog number
7163-10
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Price
Please ask
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Size
10 ug
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Synonyms
IL-28A
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Alternative_names
IL-28A
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Description
Induces antiviral defense
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Recombinant
Yes
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Source
E Coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Activity Specifications test method
Determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells. The expected ED50 is 0.3-0.5 ng/ml
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Biological activity
Determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells. The expected ED50 is 0.3-0.5 ng/ml
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Molecular Weight
19.6 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Acetic Acid.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
IFN λ-1,-2, and -3 (also known as IL-29, IL-28A and IL-28B respectively) are distantly related to the IL-10 family and Interferons. All three IFN-lambdas uses a distinct receptor system composed of an IFN-λR1 subunit (also called CRF2-12) and IL-10R2 subunit (also called CRF2-14). Signaling through this receptor system induces antiviral defense similar but distinct than the type I Interferons. Recombinant human IFN-λ2 is a 19.6 kDa protein containing 174 amino acid residues.
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Amino acid sequence
PVARLHGALP DARGCHIAQF KSLSPQELQA FKRAKDALEE SLLLKDCRCH SRLFPRTWDL RQLQVRERPM ALEAELALTL KVLEATADTD PALVDVLDQP LHTLHHILSQ FRACIQPQPT AGPRTRGRLH HWLYRLQEAP KKESPGCLEA SVTFNLFRLL TRDLNCVASG DLCV
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
IFNA1, IFNA10, IFNA6, IFNA21, IFNA5, IFNA16, IFNA14, IFNA8, IFNA7, IFNA2
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Short name
IFN-λ2, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
Interferon-λ2, H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
interferon alpha 1
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Synonyms
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Synonyms name
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 10
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Synonyms
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 6
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 21
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Synonyms
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Synonyms name
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 5
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 16
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Synonyms
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 14
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 8
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Synonyms gene name
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Synonyms
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Synonyms name
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 7
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Synonyms
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
interferon alpha 2
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Synonyms
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Synonyms name
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Locus
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Discovery year
1993-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The assay of INTERFERON-GAMMA released from lymphocytes after their exposure to a specific test antigen, to check for IMMUNOLOGIC MEMORY resulting from a previous exposure to the antigen. The amount of interferon-gamma released is usually assayed by an ENZYME-LINKED IMMUNOSORBENT ASSAY.
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Tree numbers
- E01.370.225.812.453
- E05.200.812.453
- E05.478.594.475
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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