Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight550 Conjugate

• Catalog number
  MBS687987
• Price
  Please ask
• Size
  5x1 mg

• Products_type
  Secondary Antibody
• Products_short_name
  [IgG (H&L)]
• Products_name_syn
  [Donkey anti-Sheep IgG (H&L), Affinity Pure]
• Reactivity
  Sheep. Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin sheep serum proteins
• Specificity
  Based on IEP, this antibody reacts with: heavy (gamma) chains on sheep IgG, light chains on all sheep immunoglobulins
• Purity
  Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Sheep IgG.
• Form
  Lyophilized
• Concentration
  1 mg/ml (E 1% at 280 nm = 13.0)
• Storage_stability
  Store freeze-dried powder at 2-8 degree C. Shelf Life: Product is stable for up to 4 weeks at 2-8 degree C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 degree C.
• Tested_application
  Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
• Test
  A high affinity purification column was use to purify anti- IgG (H L) - Pure, DyLight550 Conjugate by MyBioSource by chromatographic size exclusion.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti- IgG (H L) - Pure, DyLight550 Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
• Latin name
  Equus africanus asinus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Gene target
  DyLight550   Conjugate  
• Short name
  anti- IgG (H L) - Pure, DyLight550 Conjugate
• Technique
  affinity, anti-, anti, Pure, IgG, antibody to, antibodies, IgGs, pure products
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Isotype
  IgG, Heavy & Light chains, IgG
• Species
  Sheep, Donkeys, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  Donkey Antibody toSheep Immunoglobulin G (heavy + light chain) - protein Pure, DyLight550 Conjugate
• Alternative technique
  antibodies, purified, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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