Protein A/G-Biotin

• Catalog number
  6506-1
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Protein A/G
• Alternative_names
  Protein A/G
• Description
  Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Binding Capacity
  Under optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
• Molecular Weight
  59.7 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized with no additives
• Reconstitution Instructions
  Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
• Background Information
  Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated

• Gene target
  Protein   A/G  
• Gene symbol
  GPRC6A, GPR162, PURG
• Short name
  Protein A/G-
• Label
  Biotin
• Alternative name
  Protein A/G-biotinilated

Gene info

• Identity
  HGNC:18510
• Gene
  GPRC6A
• Long gene name
  G protein-coupled receptor class C group 6 member A
• Synonyms gene name
  • G protein-coupled receptor, family C, group 6, member A
  • G protein-coupled receptor, class C, group 6, member A
• Synonyms
  • bA86F4.3
• GenBank acession
  • AF502962
• Locus
  6q22.1
• Discovery year
  2002-04-26
• Entrez gene record
  222545
• Classification
  • Calcium sensing receptors
• VEGA ID
  OTTHUMG00000015447

Gene info

• Identity
  HGNC:16693
• Gene
  GPR162
• Long gene name
  G protein-coupled receptor 162
• Synonyms
  • A-2
  • GRCA
• GenBank acession
  • U47928
  • U47929
  • U47924
  • U47925
• Locus
  12p13
• Discovery year
  2005-05-09
• Entrez gene record
  27239
• Pubmed identfication
  • 15777626
• RefSeq identity
  • NM_019858
• Classification
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000168368

Gene info

• Identity
  HGNC:17930
• Gene
  PURG
• Long gene name
  purine rich element binding protein G
• Synonyms
  • PURG-A
  • PURG-B
• GenBank acession
  • AF195513
• Locus
  8p12
• Discovery year
  2004-02-09
• Entrez gene record
  29942
• Pubmed identfication
  • 12034829
• RefSeq identity
  • NM_013357
• VEGA ID
  OTTHUMG00000157357

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