HAGH, human recombinant

• Catalog number
  7832-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
• Alternative_names
  Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
• Description
  A thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is > 1.8 unit/ml, in which one unit will hydrolyze 1.0 µmole of S-lactoylglutathione per minute at pH 7.5 at 25ºC.
• Results
  Specific activity is > 1.8 unit/ml
• Unit Definition
  One unit will hydrolyze 1.0 µmole of S-lactoylglutathione per minute at pH 7.5 at 25ºC.
• Molecular Weight
  31.4 kDa (284 aa, 1-260 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
• Background Information
  HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  HAGH  
• Gene symbol
  HAGH
• Short name
  HAGH, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  HAGH, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4805
• Gene
  HAGH
• Long gene name
  hydroxyacylglutathione hydrolase
• Synonyms gene name
  • hydroxyacyl glutathione hydrolase
• Synonyms
  • GLO2
  • GLXII
  • HAGH1
• GenBank acession
  • X90999
• Locus
  16p13.3
• Discovery year
  2001-06-22
• Entrez gene record
  3029
• Pubmed identfication
  • 3025077
  • 7327557
• RefSeq identity
  • NM_005326
• Classification
  • MBL domain containing glyoxalase 2 subfamily
• VEGA ID
  OTTHUMG00000128662

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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