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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
IRAK4(Thr345)
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Specificity
This antibody reacts specifically with IRAK4(Thr345)
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Modification
Phosphorylation
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Modification site
Thr345
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
Cytoplasm
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Antigen Source
KLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345
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Gene ID
51135
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Swiss Prot
Q9NWZ3
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
IPD1; REN64; IRAK-4; NY-REN-64; Interleukin-1 receptor-associated kinase 4; Renal carcinoma antigen NY-REN-64; IRAK4
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Also known as
IRAK4(Thr345) Polyclonal Antibody
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Other name
Anti-IRAK4(Thr345) Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
IRAK4
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Short name
Anti-IRAK4(Thr345)
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 594
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Alternative name
IRAK4(Thr345) Polyclonal Antibody , ALEXA FLUOR® 594 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
interleukin-1 receptor-associated kinase 4, IRAK4 and IDBG-28022 and ENSG00000198001 and 51135, transferase activity, Extracellular, Irak4 and IDBG-177236 and ENSMUSG00000059883 and 266632, IRAK4 and IDBG-633310 and ENSBTAG00000021105 and 533692
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