Pig Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit

  • Catalog number
    EKU04862
  • Price
    Please ask
  • Size
    96 Tests
  • Estimated Turnaround Time
    6-11 Business days
  • Target Name
    Hypoxia Inducible Factor 1 Alpha
  • Target s alterntive name
    HIF1-A, MOP1, PASD8, Basic Helix-Loop-Helix Transcription Factor, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78
  • Species Reactivity
    Sus scrofa; Porcine (Pig)
  • Sample Type
    Serum, Plasma, Tissue homogenates and other biological fluids
  • Detection Range
    15.62-1000 pg/mL
  • Sensitivity
    5.5 pg/mL
  • Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
  • Detection Method
    Colorimetric
  • Assay Time
    3 hours
  • Application
    For research use only. Not for diagnostic procedures.
  • Assay Type
    Double-antibody Sandwich
  • Shipping Conditions
    Ice packs
  • Storage Temperature
    Short term: 4°C; Long term: see manual.
  • Precaution of Use
    The Stop Solution is acidic. Do not allow to contact skin or eyes.
  • Shelf life
    Use Pig Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit before 8 months
  • Specificity
    This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha
  • Cross Activity
    No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha and analogues was observed.
  • Test Principle
    No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha and analogues was observed.
  • Stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • Quality Systems
    The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research Area
    Tumor immunity, Cardiovascular biology, Reproductive science
  • Description
    The Hypoxia Factor 1 Alpha (HIF1a) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • About
    Pigs and the smaller guinea pigs are frequent used as models for humans.
  • Gene target
    Hypoxia   Factor   Alpha   HIF1a  
  • Gene symbol
    HIF1A
  • Short name
    Hypoxia Factor 1 Alpha (HIF1a) ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Pig, Pigs and Guinea pigs
  • Alternative name
    swine Hypoxia Inducible Factor 1 a (hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)) Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), bHLHe78 and HIF-1A and HIF-1alpha and HIF1 and HIF1-ALPHA and MOP1 and PASD8, HIF1A and IDBG-8510 and ENSG00000100644 and 3091, Hsp90 protein binding, nuclei, Hif1a and IDBG-148620 and ENSMUSG00000021109 and 15251, HIF1A and IDBG-646757 and ENSBTAG00000020935 and 281814
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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