PubMed number
Refer to PubMed
Net weight (g)
0.1
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's reactivity
Human
Warning information
Non-hazardous
Raised in
Rabbit
NCBI number
NP_004827
Gene number
9451
Released date
29/Aug/2016
Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr892 of human PERK (AA889-895)
Antibody's other name
Eif2ak3 Antibody, Eukaryotic translation initiation factor 2 alpha kinase 3 Antibody, Heme regulated EIF2 alpha kinase Antibody, HRI Antibody, HsPEK Antibody, Pancreatic eIF2-alpha kinase Antibody, PEK Antibody, PRKR-like Endoplasmic Reticulum kinase Antibody, WRS Antibody
Protein number
Q9NZJ5
Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
Tested applications
To be tested
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Total weight (kg)
1.4
Stock availabilit
In Stock
Scientific context
PERK (eukaryotic translation initiation factor 2-α kinase 3, eIF2α kinase 3, PEK, WRS) is a Ser/Thr protein kinase, type I membrane protein of the endoplasmic reticulum that is abundant in secretory tissues and is inducible by ER-stress. PERK phosphorylates the α subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, a reduction of translation initiation and repression of global protein synthesis. PERK may serve as an effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. PERK dimerizes with HSPA5/BIP in resting cells and oligomerizes in ER-stressed cells. Perturbation in protein folding in the endoplasmic reticulum (ER) can promote dissociation from HSPA5/BIP and oligomerization, resulting in autophosphorylation and kinase activity induction.
Antibody's target
Human PERK (pThr892)
Certificate of analysis
A 1:250 dilution of SPC-963 was sufficient for detection of PERK (pThr892) in 10 µg of HepG2 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Tissue specificity
See included datasheet or contact our support service.
Antibody's full description
Rabbit Anti-Human PERK (pThr892) Polyclonal
Country of production
Canada
Antibody's purified from
Peptide Affinity Purified
Antibody's concentration
See included datasheet or contact our support service
Tested reactivity
To be tested
Antibody in cell
Endoplasmic Reticulum Membrane
Antibody's category
Polyclonal Antibodies
Representative figure link
No Data Available
Bibliography
Refer to PubMed
Verified applications
WB, AM
Antibody's specificity
Detects 125.147 kDa.
Storage recommendations
-20°C
Shipping recommendations
Blue Ice or 4°C
