Mouse anti HLA-DR

• Catalog number
  MUB2051P
• Price
  Please ask
• Size
  0,5mL

• Category
  Primary Antibodies
• Long description
  The major histocompatibility complex (MHC) is a cluster of genes that play an important role in the immune response. In humans, this complex is referred to as the Human Leucocyte Antigen (HLA) system and is located on chromosome 6. In humans the MHC II locus includes genes for alpha and beta subunits of the three class II MHC proteins HLA-DR, HLA-DQ and HLA-DP. The HLA class II alpha and beta genes are highly polymorphic with the exception of HLA-DR alpha gene, which is reported to be monomorphorphic. HLA class II glycoproteins are expressed as alpha/beta heterodimers on the surface of antigen presenting cells such as dendritic cells, B-cells and monocytes. Their primary function is to present foreign antigen derived peptides to CD4 positive T-cells. HLA-class II molecules also play an important role in developing and maintaining tolerance to self-antigens and in transplant rejection. HLA-DR molecules have been associated with a range of autoimmune diseases including rheumatoid arthritis, Grave’s disease and multiple sclerosis.
• Antibody come from
  Bra30 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with cells from a mouse immunized with REH-6 cells, a human pre-B cell leukaemia cell line.
• Other description
  Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
• Clone
  Bra30
• Antigen antibody binding interaction
  Mouse anti HLA-DR Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  The antibody Bra30 is directed against a monomorphic determinant of human HLA Class II. The antibody recognises an epitope on the HLA-DR molecule that is dependent on the association of both heavy and lights chains of the HLA class II molecule. The antibody does not cross react with either HLA-DP or HLA-DQ molecules. The antibody has not been tested for cross reactivity with other species.
• Research interest
  Immunology
• Application
  Flow Cytometry, Immunocytochemistry, Immunohistology (frozen), Immunoprecipitation, Western Blotting
• Antibody s suited for
  The Bra30 antibody is suitable for the detection of HLA Class II antigen by flow cytometry, immunocytochemistry and immunohistochemistry on frozen tissues. The antibody is also suitable for the detection of HLA class II by immunoprecipitation and western blotting. Optimal antibody dilutions for the different applications should be determined by titration.
• Storage
  Store at 4oC, or in small aliquots at -20oC.
• Relevant references
  1. Poláková K, Karpatová M.(1990).Study of monomorphic determinants on DR molecules of HLA class II antigens. Neoplasma. 37:239-51. 2. Lahat N, Bitterman H, Weiss-Cerem L, Rahat MA.(2011). Hypoxia increases membranal and secreted HLA-DR in endothelial cells, rendering them T-cell activators.Transpl. Int. 24:1018-26.
• Protein number
  UniProt: P01903
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  HLA-DR  
• Gene symbol
  HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DPB1, HLA-DQA2, HLA-DQB2, HLA-W, HLA-DRA, HLA-S
• Short name
  Mouse anti HLA-DR
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG2a
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Human leukocyte antigen-DR
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:4939
• Gene
  HLA-DPA2
• Long gene name
  major histocompatibility complex, class II, DP alpha 2 (pseudogene)
• Synonyms gene
  • HLA-DP2A
• GenBank acession
  • X01634
• Locus
  6p21.32
• Discovery year
  1986-01-01
• Entrez gene record
  646702
• Pubmed identfication
  • 6597088
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031061
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4936
• Gene
  HLA-DOA
• Long gene name
  major histocompatibility complex, class II, DO alpha
• Synonyms gene
  • HLA-DZA
  • HLA-DNA
• Synonyms
  • HLA-D0-alpha
• GenBank acession
  • M31525
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3111
• Pubmed identfication
  • 2370084
• RefSeq identity
  • NM_002119
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031211
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4942
• Gene
  HLA-DQA1
• Long gene name
  major histocompatibility complex, class II, DQ alpha 1
• Synonyms gene
  • HLA-DQA
• Synonyms
  • CELIAC1
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3117
• RefSeq identity
  • NM_002122
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031106
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:19406
• Gene
  HLA-N
• Long gene name
  major histocompatibility complex, class I, N (pseudogene)
• Synonyms
  • HLA-30
• GenBank acession
  • M96329
• Locus
  6p22.1
• Discovery year
  2002-10-23
• Entrez gene record
  267014
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009940
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031167
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4940
• Gene
  HLA-DPB1
• Long gene name
  major histocompatibility complex, class II, DP beta 1
• Synonyms gene
  • HLA-DP1B
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3115
• RefSeq identity
  • NM_002121
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031076
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4943
• Gene
  HLA-DQA2
• Long gene name
  major histocompatibility complex, class II, DQ alpha 2
• Synonyms gene
  • HLA-DXA
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3118
• RefSeq identity
  • NM_020056
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031108
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4945
• Gene
  HLA-DQB2
• Long gene name
  major histocompatibility complex, class II, DQ beta 2
• Synonyms gene
  • HLA-DXB
• GenBank acession
  • M83890
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3120
• Pubmed identfication
  • 2564844
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031125
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:23425
• Gene
  HLA-W
• Long gene name
  major histocompatibility complex, class I, W (pseudogene)
• Synonyms gene
  • HLA-80
• Synonyms gene name
  • HLA-80 pseudogene
• GenBank acession
  • M96334
• Locus
  6p22.1
• Discovery year
  2004-01-16
• Entrez gene record
  352966
• Pubmed identfication
  • 10557312
  • 1517563
• RefSeq identity
  • NG_015995
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031242
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:4947
• Gene
  HLA-DRA
• Long gene name
  major histocompatibility complex, class II, DR alpha
• Synonyms gene
  • HLA-DRA1
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3122
• RefSeq identity
  • NM_019111
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031269
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:19395
• Gene
  HLA-S
• Long gene name
  major histocompatibility complex, class I, S (pseudogene)
• Synonyms
  • HLA-17
• GenBank acession
  • M96339
• Locus
  6p21.33
• Discovery year
  2002-10-23
• Entrez gene record
  267015
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009941
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031178
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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