Polystyrene Particle Size Standard Kit, Flow Cytometry Grade, 2.5E6/mL, 6x5mL, 2.0-2.4µm, 3.0-3.4µm, 5.0-5.9µm, 7.0-7.9µm, 8.0-12.9µm, & 13.0-17.9µm

• Catalog number
  PPS-6K
• Price
  Please ask
• Size
  1 unit

• Notes
  For research use only.
• Additional description
  FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

• Gene target
  Polystyrene   Particle   Size   Kit   Grade   5E6   6x5mL   0-2   4µm   0-3   4µm   0-5   9µm   0-7   9µm   0-12   9µm   0-17   9µm  
• Gene symbol
  IGKV1OR2-0, SNORD112, H1-0, IGHVIII-5-2, MIR7-3, TRP-AGG2-7, TRG-GCC2-5, MIR7-2, KRTAP5-7
• Short name
  Polystyrene Particle Size Standard Kit, Grade, 2 5E6/ , 6x5mL, 2 -2 4µm, 3 -3 4µm, 5 -5 9µm, 7 -7 9µm, 8 -12 9µm, & 13 -17 9µm
• Technique
  Flow Cytometry, Standard, Flow, standards
• Alternative name
  Polystyrene Particle Size reference reagent, Flow Cytometry Grade, 2.5E6/milliliter, 6x5mL, 2.0-2.4µm, 3.0-3.4µm, 5.0-5.9µm, 7.0-7.9µm, 8.0-12.9µm, & 13.0-17.9µm
• Alternative technique
  standards, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:5766
• Gene
  IGKV1OR2-0
• Long gene name
  immunoglobulin kappa variable 1/OR2-0 (non-functional)
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR2-0
• Synonyms
  • IGKV1/OR2-0
• GenBank acession
  • Y08392
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28867
• Classification
  • Immunoglobulin kappa (IGK) orphons

Gene info

• Identity
  HGNC:32777
• Gene
  SNORD112
• Long gene name
  small nucleolar RNA, C/D box 112
• Synonyms
  • 14q(0)
• Locus
  14q32.31
• Discovery year
  2006-05-08
• Entrez gene record
  692215
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003080
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:4714
• Gene
  H1-0
• Long gene name
  H1.0 linker histone
• Synonyms gene
  • H1FV
  • H1F0
• Synonyms gene name
  • H1 histone family, member 0
  • H1 histone family member 0
• Synonyms
  • H1.0
• Synonyms name
  • H1.0, H1(0), H1-0
• GenBank acession
  • X03473
• Locus
  22q13.1
• Discovery year
  1988-07-20
• Entrez gene record
  3005
• Pubmed identfication
  • 3084796
• RefSeq identity
  • NM_005318
• Classification
  • H1 histones
• VEGA ID
  OTTHUMG00000150659

Gene info

• Identity
  HGNC:5703
• Gene
  IGHVIII-5-2
• Long gene name
  immunoglobulin heavy variable (III)-5-2 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-5-2
  • immunoglobulin heavy variable (III)-5-2 pseudogene
• Synonyms
  • IGHV(III)-5-2
• GenBank acession
  • AB019440
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28353
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152278

Gene info

• Identity
  HGNC:31640
• Gene
  MIR7-3
• Long gene name
  microRNA 7-3
• Synonyms gene
  • MIRN7-3
• Synonyms
  • hsa-mir-7-3
• Locus
  19p13.3
• Discovery year
  2004-04-23
• Entrez gene record
  407045
• RefSeq identity
  • NR_029607
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:34698
• Gene
  TRP-AGG2-7
• Long gene name
  tRNA-Pro (anticodon AGG) 2-7
• Synonyms gene
  • TRNAP9
  • TRP-AGG2-5
• Synonyms gene name
  • transfer RNA proline 9 (anticodon AGG)
  • transfer RNA-Pro (AGG) 2-5
  • transfer RNA-Pro (AGG) 2-7
• Synonyms
  • tRNA-Pro-AGG-2-7
• GenBank acession
  • HG983982
• Locus
  16p13.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189136
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34725
• Gene
  TRG-GCC2-5
• Long gene name
  tRNA-Gly (anticodon GCC) 2-5
• Synonyms gene
  • TRNAG16
  • TRG-GCC2-3
• Synonyms gene name
  • transfer RNA glycine 16 (anticodon GCC)
  • transfer RNA-Gly (GCC) 2-3
  • transfer RNA-Gly (GCC) 2-5
• Synonyms
  • tRNA-Gly-GCC-2-5
• GenBank acession
  • HG983813
• Locus
  16q22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189163
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:23602
• Gene
  KRTAP5-7
• Long gene name
  keratin associated protein 5-7
• Synonyms
  • KRTAP5.7
  • KRTAP5-3
• GenBank acession
  • AB126076
• Locus
  11q13.4
• Discovery year
  2003-12-01
• Entrez gene record
  440050
• Pubmed identfication
  • 15144888
• Classification
  • Keratin associated proteins
• VEGA ID
  OTTHUMG00000057570

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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