IMPAD1, human recombinant

• Catalog number
  7812-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3
• Alternative_names
  Inositol monophosphatase 3, GPAPP, IMP 3, IMP-3, IMPA3
• Description
  Catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP).
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity > 3300 pmole/min/µg, its ability to dephosphorylate adenosine 3’5’-diphosphate sodium salt at pH 7.5, 25ºC.
• Results
  Specific activity > 3300 pmole/min/µg
• Molecular Weight
  37.6 kDa (349 aa, 34-359 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.25 mg/ml
• Appearance
  Liquid
• Physical form description
  0.25 mg/ml solution in PBS (pH 7.4).
• Background Information
  IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  IMPAD1  
• Gene symbol
  BPNT2
• Short name
  IMPAD1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  IMPAD1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:26019
• Gene
  BPNT2
• Long gene name
  3'(2'), 5'-bisphosphate nucleotidase 2
• Synonyms gene
  • IMPAD1
• Synonyms gene name
  • inositol monophosphatase domain containing 1
• Synonyms
  • FLJ20421
  • IMPA3
  • gPAPP
• Synonyms name
  • golgi-resident nucleotide phosphatase
  • Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase
• Locus
  8q12.1
• Discovery year
  2005-08-02
• Entrez gene record
  54928
• Pubmed identfication
  • 21549340
  • 22289690
  • 18695242
  • 24799377
• RefSeq identity
  • NM_017813
• VEGA ID
  OTTHUMG00000164415

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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