Anti-SIGLEC10 (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-2706R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideSIGLEC10
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SpecificityThis antibody reacts specifically with SIGLEC10
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsExtracellular
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Antigen SourceKLH conjugated synthetic peptide derived from human SIGLEC10
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Gene IDN/A
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Swiss ProtN/A
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ApplicationsFCM, IF(IHC-P)
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Applications with corresponding dilutionsFCM(1:20-100), IF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationSIGLEC10 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SIGLEC10 interacts with PTPN6/SHP-1 upon phosphorylation. The protein is expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation).
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsPRO940; mSiglec G; Sialic acid binding Ig like lectin 10; Sialic acid binding Ig like lectin 10; sialic acid binding Ig like lectin 10 Ig like lectin 7; sialic acid binding Ig like lectin G; Sialic acid binding Immunoglobulin like lectin 10; SIGLEC 10; Siglec G; siglec like gene 2; Siglec like protein 2; Siglec like protein 2; Siglecg; SLG2.
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Also known asSIGLEC10 Polyclonal Antibody
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Other nameAnti-SIGLEC10 Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolSIGLEC10-AS1, SIGLEC10
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Short nameAnti-SIGLEC10
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameSIGLEC10 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Alternative to gene targetsialic acid binding Ig-like lectin 10, SIGLEC10 and IDBG-65888 and ENSG00000142512 and 89790, protein binding, Extracellular
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Gene info
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Identity
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Gene
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Long gene nameSIGLEC10 antisense RNA 1
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Locus
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Discovery year2020-03-20
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene namesialic acid binding Ig like lectin 10
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Synonyms gene name
- sialic acid binding Ig-like lectin 10
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2-set domain containing
- Sialic acid binding Ig like lectins
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data