EzH2 polyclonal antibody

• Catalog number
  6838-25
• Price
  Please ask
• Size
  25 μg

• Antibody Target
  EzH2
• Target Alternative Name
  Enhancer of zeste homolog 2
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse.
• Immunogen Sequence
  Polyclonal antibody raised in rabbit against the N-terminus (aa1-343) of the mouse EZH2 protein (Enhancer of zeste homolog 2).
• Accession
  Q15910
• Gene Id
  2146
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.05% sodium azide and 0.05% ProClin 300.
• Purification
  Protein G purified
• Application
  Western blot, ChIP, IHC.
• Positive Control
  Western blot: HeLa cells, ChIP: HeLa cells, IHC.
• Application And Usages
  ChIP: 2.5 µg/ChIP, Western Blot: 1:1000, IHC: 1:100 – 1:500.
• Description
  EZH2 is a histone-lysine methyltransferase which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  EzH2  
• Gene symbol
  PCSEAT, TMPOP2, EZH2
• Short name
  EzH2 polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Rabbit IgG
• Alternative name
  EzH2 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:54485
• Gene
  PCSEAT
• Long gene name
  prostate cancer expressed EZH2 associated transcript
• Synonyms
  • PRCAT38
• Synonyms name
  • prostate cancer-associated transcript 38
  • PCa specific expression and EZH2-associated transcript
• Locus
  21q22.3
• Discovery year
  2019-09-12
• Pubmed identfication
  • 29671889
  • 29803673
• Classification
  • Long non-coding RNAs with non-systematic symbols

Gene info

• Identity
  HGNC:50860
• Gene
  TMPOP2
• Long gene name
  thymopoietin pseudogene 2
• Synonyms gene name
  • thymopoietin pseudogene 2 (functional)
• Synonyms
  • lncRNA-EBIC
• Synonyms name
  • EZH2-binding lncRNA in cervical cancer
• Locus
  16q23.1
• Discovery year
  2014-07-06
• Entrez gene record
  100533619
• Pubmed identfication
  • 25007342
• RefSeq identity
  • NG_028880
• VEGA ID
  OTTHUMG00000177355

Gene info

• Identity
  HGNC:3527
• Gene
  EZH2
• Long gene name
  enhancer of zeste 2 polycomb repressive complex 2 subunit
• Synonyms gene name
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste homolog 2 (Drosophila)
• Synonyms
  • EZH1
  • ENX-1
  • KMT6
  • KMT6A
• Locus
  7q36.1
• Discovery year
  1995-12-21
• Entrez gene record
  2146
• Pubmed identfication
  • 8954776
  • 17172412
• RefSeq identity
  • NM_004456
• Classification
  • SET domain containing
  • Polycomb repressive complex 2
  • Lysine methyltransferases
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000158973
• Locus Specific Databases
  • LRG_531

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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