Rabbit Anti-Human MMP 1 Polyclonal: PE/ATTO 594

  • Catalog number
    SPC-1277D-P594
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A synthesized peptide derived from human MMP1
  • Antibody s target
    Human MMP 1
  • Antibody s full description
    Rabbit Anti-Human MMP 1 Polyclonal
  • Primary research fields
    Cardiovascular System, Angiogenesis, Growth Factors, Adhesion / Extracellular Matrix, Matrix Metalloproteinases, Cell Signaling, Extracellular Matrix (ECM), Enzymes, Cancer, Invasion/microenvironment
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    27 kDa interstitial collagenase Antibody, CLG Antibody, CLGN Antibody, Matrix metallopeptidase 1 (interstitial collagenase) Antibody, Matrix metalloprotease 1 Antibody, MMP 1 Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:3000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~54kDa, endogenous levels of total MMP1
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1277 was sufficient for detection of MMP 1 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Extracellular matrix
  • Tissue specificity
    Expressed in fibroblasts, keratinocytes, endothelial cells, monocytes, macrophages
  • Scientific context
    Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the ability to degrade all the components of the extracellular matrix. MMP1 in particular cleaves collagen types I, II, III and IV, as well as casein, gelatin, antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. MMP1 is downregulated by p53, and abnormality of p53 expression may lead to joint degradation in rheumatoid arthritis.
  • Bibliography
    1. Cawston T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_002412.1
  • Gene number
    4312
  • Protein number
    P03956
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Human HepG2 cell lysates showing detection of ~54kDa MMP 1 protein using Rabbit Anti-MMP 1 Polyclonal Antibody (SPC-1277). Lane 1: Human HepG2 cell lysate treated with the immunizing peptide. Lane 2: Human HepG2 cell lysate. Primary Antibody: Rabbit Anti-MMP 1 Polyclonal Antibody (SPC-1277) at 1:1000. Predicted/Observed Size: ~54kDa. Rabbit Anti-MMP 1 Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1277)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    MMP   PE/ATTO   594  
  • Gene symbol
    MMP24, MMP14, MMP28, MMP17, MMP15, MMP25, MMP16
  • Short name
    Rabbit Anti- MMP 1 Polyclonal: PE/ATTO 594
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG
  • Label
    PE/ATTO 594
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman MMP 1 polyclonal: peroxidase/ATTO 594
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    matrix metallopeptidase 17
  • Synonyms gene name
    • matrix metalloproteinase 17 (membrane-inserted)
    • matrix metallopeptidase 17 (membrane-inserted)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-11-13
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • M10 matrix metallopeptidases
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    matrix metallopeptidase 16
  • Synonyms gene
  • Synonyms gene name
    • matrix metalloproteinase 16 (membrane-inserted)
    • chromosome 8 open reading frame 57
    • matrix metallopeptidase 16 (membrane-inserted)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-11-13
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • M10 matrix metallopeptidases
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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