Mouse Anti-Human SOD (EC) Monoclonal IgG1 Kappa, Clone 4GG11G6

  • Catalog number
    SMC-167D-A680
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    4GG11G6
  • Immunogen
    Human extracellular SOD purified from aortas
  • Antibody s full description
    Mouse Anti-Human SOD3 Monoclonal IgG1 Kappa Antibody, Clone: 4GG11G6: ATTO 680
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    EC SOD antibody, EC-SOD antibody, Extracellular superoxide dismutase [Cu Zn] antibody, Extracellular superoxide dismutase [Cu-Zn] antibody, Extracellular superoxide dismutase antibody, Extracellular superoxide dismutase precursor antibody, MGC20077 antibody, SOD 3 antibody, SOD3 antibody, SODE_HUMAN antibody, Superoxide dismutase 3 extracellular antibody
  • Raised in
    Mouse
  • Antibody s target
    SOD3
  • Primary research fields
    Cancer, Oxidative Stress
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, ELISA
  • Antibody s reactivity
    Human, Mouse, Rat, Guinea Pig (Cavia porcellus)
  • Antibody s dilutions
    WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects extracellular SOD ~35kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-167 was sufficient for detection of EC-SOD in 20 µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Extracellular Space
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
  • Bibliography
    1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O’Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
  • Released date
    1-May-2009
  • PubMed number
    21112335, 20887783
  • Tested applications
    Western Blot, Immunohistochemistry
  • Tested reactivity
    Guinea-pig, Human
  • NCBI number
    NP_003093.2
  • Gene number
    6649
  • Protein number
    P08294
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Western Blot analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 (SMC-167). Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody (SMC-167) at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteoarthritis-arthritis.. Mouse Anti-SOD3 Antibody [4GG11G6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human cartilage (SMC-167) Mouse Anti-SOD3 Antibody [4GG11G6] used in Western Blot (WB) on Human cartilage lysates (SMC-167)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
    SOD   Monoclonal   IgG1   Kappa   4GG11G6  
  • Gene symbol
    SOD3
  • Short name
    Mouse Anti- SOD (EC) Monoclonal IgG1 Kappa, Clone 4GG11G6
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1 Kappa, IgG1
  • Label
    ATTO 680
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman antioxidant enzyme (EC) monoclonal IgG1 Kappa, clonality 4GG11G6
  • Alternative technique
    murine, antibodies
  • Clone name
    4GG11G6
Gene info
  • Identity
  • Gene
  • Long gene name
    superoxide dismutase 3
  • Synonyms gene name
    • superoxide dismutase 3, extracellular
  • Synonyms
  • Locus
  • Discovery year
    1988-05-08
  • Entrez gene record
  • Classification
    • Superoxide dismutases
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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