Recombinant Human SerpinE1 / PAI-1 Protein (His tag) 20µg

• Catalog number
  PKSH031702-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  PAI;PAI-1;PAI1;PLANH1;SERPINE1
• Activity
  Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 60 nM.
• Sequence
  Met 1-Pro 402
• Fusion tag
  C-His
• Accession
  NP_000593.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞97 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  44.2 kDa
• AP Mol Mass
  45 kDa
• Formulation
  Lyophilized from sterile 50mM NaAc, 0.1M NaCl, pH 5.5
• Reconstitution
  Please refer to it for detailed information.
• Background
  Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SerpinE1 and PLANH1, is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. High concentrations of SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta-sheet A and formation of covalent complexes with target proteases. Greater expression of PAI-1 has been associated with increased survival of cells and resistance to apoptosis. PAI-1 appears to influence apoptosis by decreasing cell adhesion (anoikis) as well as its effect on intracellular signaling. PAI-1, in its active state, also binds to the extracellular protein vitronectin. When in complex with its target proteases, it binds with high affinity to endocytosis receptors of the low density receptor family. The mechanisms of PAI-1 overexpression during obesity are complex, and it is conceivable that several inducers are involved at the same time at several sites of synthesis. PAI-1 is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Gene
  Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitor or serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. Elevated PAI-1 is a risk factor for thrombosis and atherosclerosis
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SerpinE1   PAI-1   Protein   His   tag   20µg  
• Gene symbol
  SERPINE1, SERPINB2, SERBP1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1
• Short name
  Recombinant SerpinE1 / PAI-1 Protein (His tag) 20µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor classification 1), member 1 / PAI-1 Protein (histidine detection labelled) 20µg
• Alternative technique
  rec
• Alternative to gene target
  serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, PAI and PAI-1 and PAI1 and PLANH1, SERPINE1 and IDBG-32959 and ENSG00000106366 and 5054, protein binding, Extracellular, Serpine1 and IDBG-204786 and ENSMUSG00000037411 and 18787, SERPINE1 and IDBG-640415 and ENSBTAG00000014465 and 281375

Gene info

• Identity
  HGNC:8583
• Gene
  SERPINE1
• Long gene name
  serpin family E member 1
• Synonyms gene
  • PLANH1
  • PAI1
• Synonyms gene name
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Synonyms
  • PAI
• Synonyms name
  • plasminogen activator inhibitor, type I
• GenBank acession
  • M16006
• Locus
  7q22.1
• Discovery year
  1986-01-01
• Entrez gene record
  5054
• Pubmed identfication
  • 3097076
  • 2891140
  • 24172014
• RefSeq identity
  • NM_000602
• Classification
  • Serpin peptidase inhibitors
• VEGA ID
  OTTHUMG00000157107
• Locus Specific Databases
  • LRG_597

Gene info

• Identity
  HGNC:8584
• Gene
  SERPINB2
• Long gene name
  serpin family B member 2
• Synonyms gene
  • PLANH2
  • PAI2
• Synonyms gene name
  • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2
  • serpin peptidase inhibitor, clade B (ovalbumin), member 2
• Synonyms
  • HsT1201
  • PAI-2
• Synonyms name
  • plasminogen-activator inhibitor
• GenBank acession
  • Y00630
• Locus
  18q21.33-q22.1
• Discovery year
  1989-02-07
• Entrez gene record
  5055
• Pubmed identfication
  • 24172014
• RefSeq identity
  • NM_002575
• Classification
  • Serpin peptidase inhibitors
• VEGA ID
  OTTHUMG00000060592

Gene info

• Identity
  HGNC:17860
• Gene
  SERBP1
• Long gene name
  SERPINE1 mRNA binding protein 1
• Synonyms
  • PAI-RBP1
  • DKFZP564M2423
  • CGI-55
  • HABP4L
  • PAIRBP1
  • CHD3IP
• GenBank acession
  • AF151813
• Locus
  1p31.3
• Discovery year
  2005-09-15
• Entrez gene record
  26135
• Pubmed identfication
  • 11001948
  • 10810093
  • 18440126
  • 17698176
• RefSeq identity
  • NM_001018067
• VEGA ID
  OTTHUMG00000009372

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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