Glucose-1-Phosphate Colorimetric Assay Kit
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Catalog numberK697-100
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PricePlease ask
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Size100 assays
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Summary• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Application- The kit can detect glucose-1-phosphate concentrations in the range of 1 µM – 10 mM.
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Sample Type• Animal tissues: Liver, muscle and heart etc. • Cell culture: Adherent or suspension cells.
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Features Benefits• Simple procedure; takes ~ 40 minutes • Rapid, convenient and sensitive • Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundGlucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P G1P), UDPG-pyrophosphorylase (G1P UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.
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TestBiovision supplies other types of Assays as 1.
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PropertiesColorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbolIGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1, PIRC1
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Short nameGlucose-1-Phosphate Colorimetric Assay Kit
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Techniquecolorimetric, Assay, assays, colorimetry
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HostAssay
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Alternative nameGlucose-1-Phosphate colorimetry test reagent
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Alternative techniqueassays, arrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR101 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 16-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR15/16 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 1
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters