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Alternative name 1
CD19 (Clone:HD37), RPE-Cy5 + Kappa, FITC [Pab F(ab')2] + Lambda, RPE [Pab F(ab')2], Human, tri-label
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Alternative name 2
CD19 (Clone:HD37), RPE-Cy5 + Kappa, FITC [Pab F(ab')2] + Lambda, RPE [Pab F(ab')2], Human, tri-label
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Alternative name 3
CD19 (Clone:HD37), RPE-Cy5 + Kappa, FITC [Pab F(ab')2] + Lambda, RPE [Pab F(ab')2], Human, tri-label
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Alternative name 4
anti-CD19 Clone:HD37 + Kappa [Pab F ab' 2] + Lambda [Pab F ab' 2] tri-label
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Alternative name 5
CD19 Clone:HD37 + Kappa [Pab F ab' 2] + Lambda [Pab F ab' 2] tri-label
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Cy5.5, Anti-FITC Antibody
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Group
f(ab')2s
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Additional description
Brady printing labels are printed by a label printer for labeling with 6,5 m long cassettes.
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Gene target
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Gene symbol
RPE, CD19
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Short name
CD19 (Clone:HD37), RPE- + Kappa, FITC [Pab F(ab')2] + Lambda, RPE [Pab F(ab')2], , tri-
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Technique
F(ab')2, FITC, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. Fluorescein
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Isotype
F(ab')2 Fragment Affinity-Purified Secondary Antibodies
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Label
rpe
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Species
Human, Humans
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Alternative name
CD19 molecule (clonality:HD37), RPE-cyanine 5 + Kappa, fluorecein [polyclonal F(Antibody')2] + Lambda, RPE [polyclonal F(Antibody')2], H. sapiens, tri-label
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Alternative technique
secondairy, fluorescine
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Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
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