EZCut™ SUMO Protease 1 Agarose Beads
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Catalog number
M1028-5
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Price
Please ask
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Size
5 ml
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Description
EZCut™ SUMO Protease 1 Agarose is prepared by covalent coupling of SUMO Protease 1 to activated 4% cross-linked agarose beads.. SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. EZCut™ SUMO Protease 1 Agarose is prepared by covalent coupling of SUMO Protease 1 to activated 4% cross-linked agarose beads. EZCut™ SUMO Protease 1 Agarose Beads are designed for efficient cleavage of recombinant fusion proteins, containing a SUMO tag, circumventing the need for additional chromatographic step(s) to remove the protease after cleavage reaction is completed. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. These beads can be regenerated for repeated use and the reusability depending on the usage and handling of the product.
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Product Highlights
EZCut™ SUMO Protease 1 Agarose Beads are designed for efficient cleavage of recombinant fusion proteins, containing a SUMO tag, circumventing the need for additional chromatographic step(s) to remove the protease after cleavage reaction is completed.
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
6 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
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Properties
The purest agarose was used in the production of EZCut™ SUMO Protease 1 Agarose by Biovision.
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Conjugation
Agarose
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Gene target
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Gene symbol
SUMO1
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Short name
EZCut™ SUMO Protease 1 Agarose
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Technique
Agarose, agaroses, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet.
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Label
Agarose
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Alternative name
EZCut™ SUMO Protease 1 molecular sieve particles
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Alternative technique
separation
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
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Tree numbers
- E05.196.401.153
- E05.301.300.100
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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