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Availability
Please contact us to check the availability
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Immunogen
Synthetic peptide corresponding to human RAGE (42-59)
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Specificity
Human Receptor for Advanced Glycation End Products
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Species reactivity
Human, cross-reactivity with mouse, rat and bovine receptor expected
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Product presentation
Serum containing 0.01% Merthiolate (Thimerosal)
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Product category
Primary Antibody
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Antibody s raised in
Goat
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Antibody s isotype
Serum
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
IHC (P) (1:400), ELISA (1:10000-1:260000), WB 1:2000
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Protein concentration
N/A
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Positive control
human renal tissue
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Pretreatment
In immunohistochemistry of formaldehyde-fixed paraffin embedded tissue we recommend high temperature antigen unmasking with citrate buffer (Art. No. DE000) 15-30 min.
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Tested applications
IHC(P), ELISA , WB
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Recommended secondary reagent
We recommend the use of anti-goat IgG:Biotin (Art. No. BA-9500) in combination with Streptavidin:HRPO (Art. No. SA-5004) and a suitable chromogenic substrate e.g. DAB (Art. No. DA002) or AEC (Art. No. AE002).
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Storage method
Store at -20°C
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UniProt number
Q151Go to UniProt/SwissProt9 (RAGE_HUMAN)
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Litterature
1. Schmidt, A.M., Hori O., Cao R., Yan S.D., Brett J., Wautier J.L., Ogawa S., Kuwabara K., Matsumoto M., and Stern D. (1996) RAGE: a novel cellular receptor for advanced glycation end products. Diabetes 45 Suppl. 3; S 77-80 Review.
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Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** Merthiolate (Thimerosal) is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low Merthiolate concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Latin name
Capra aegagrus hircus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies, Sera
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Serum should be stored at +5°C
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Gene target
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Gene symbol
AGER, MOK
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Short name
AGE
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Technique
Polyclonal, anti-, serum, anti, Goat, antibody to, antibodies, goats, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Species
Human, Goats, Humans
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Alternative name
RAGE, human, Receptor for Advanced Glycation End Products
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Alternative technique
polyclonals, sera, antibodies, caprine
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Tissue
serum
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