Synthetic peptide corresponding to human RAGE (42-59)
Human Receptor for Advanced Glycation End Products
Human, cross-reactivity with mouse, rat and bovine receptor expected
Serum containing 0.01% Merthiolate (Thimerosal)
Recommended concentration for use
IHC (P) (1:400), ELISA (1:10000-1:260000), WB 1:2000
human renal tissue
In immunohistochemistry of formaldehyde-fixed paraffin embedded tissue we recommend high temperature antigen unmasking with citrate buffer (Art. No. DE000) 15-30 min.
IHC(P), ELISA , WB
Recommended secondary reagent
We recommend the use of anti-goat IgG:Biotin (Art. No. BA-9500) in combination with Streptavidin:HRPO (Art. No. SA-5004) and a suitable chromogenic substrate e.g. DAB (Art. No. DA002) or AEC (Art. No. AE002).
Store at -20°C
Q151Go to UniProt/SwissProt9 (RAGE_HUMAN)
1. Schmidt, A.M., Hori O., Cao R., Yan S.D., Brett J., Wautier J.L., Ogawa S., Kuwabara K., Matsumoto M., and Stern D. (1996) RAGE: a novel cellular receptor for advanced glycation end products. Diabetes 45 Suppl. 3; S 77-80 Review.
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** Merthiolate (Thimerosal) is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low Merthiolate concentration it must be handled with care. Dispose according to regional rules!
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Capra aegagrus hircus
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies,Sera
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Serum should be stored at +5°C