Mouse Monoclonal IgG1 Anti-Cytokeratin 10 [DE-K10]

  • Catalog number
    CK110
  • Price
    Please ask
  • Size
    1 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Extract of human epidermis
  • Specificity
    56,5 kDa Cytokeratin according to CK10 (Moll et al. 1982)
  • Species reactivity
    Human, cattle, rat
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
  • Product category
    Primary Antibody
  • Antibody s raised in
    Mouse
  • Antibody s isotype
    IgG1
  • Antibody s clone
    DE-K10
  • Product is supplied as
    liquid concentrate
  • Recommended concentration for use
    1:10 - 1:50
  • Protein concentration
    N/A
  • Positive control
    Squamous epithelial carcinoma (Vulva)
  • Pretreatment
    Unmasking fluid C (Art. No. DE000) or unmasking fluid G (Art. No. DE007) at high temperature is recommended for the use on formaldehyde fixed tissue.
  • Tested applications
    IHC(C, P)
  • Recommended secondary reagent
    We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Ivanyi D., Ansink A., Groeneveld E., Hageman P.C., Mooi W.J., and Heintz A.P.M. (1989) New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J. Pathol. 159; 7-12. 3. Ivanyi D., Groeneveld E., Van Doornewaard G., Mooi W.J., and Hageman P.C. (1990) Keratin subtypes in carcinomas of the uterin cervix: implications for histiogenesis and differential diagnosis. Cancer Res. 50; 5143-5152. 4. Mommers JM, van Rossum MM, van Erp PE, van De Kerkhof PC. (2000) Changes in keratin 6 and keratin 10 (co-)expression in lesional and symptomless skin of spreading psoriasis. Dermatology 2000;201(1):15-20
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    KRT10, DSEL, TRHDE
  • Short name
    Bioprime
  • Technique
    Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG1
  • Species
    Mouse, Mouses
  • Alternative name
    Cytokeratin 10
  • Alternative technique
    murine, antibodies
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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