Mouse Monoclonal IgG1 Anti-Cytokeratin 10 [DE-K10]
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Catalog numberCK110
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PricePlease ask
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Size1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenExtract of human epidermis
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Specificity56,5 kDa Cytokeratin according to CK10 (Moll et al. 1982)
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Species reactivityHuman, cattle, rat
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG1
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Antibody s cloneDE-K10
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Product is supplied asliquid concentrate
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Recommended concentration for use1:10 - 1:50
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Protein concentrationN/A
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Positive controlSquamous epithelial carcinoma (Vulva)
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PretreatmentUnmasking fluid C (Art. No. DE000) or unmasking fluid G (Art. No. DE007) at high temperature is recommended for the use on formaldehyde fixed tissue.
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Tested applicationsIHC(C, P)
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Ivanyi D., Ansink A., Groeneveld E., Hageman P.C., Mooi W.J., and Heintz A.P.M. (1989) New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J. Pathol. 159; 7-12. 3. Ivanyi D., Groeneveld E., Van Doornewaard G., Mooi W.J., and Hageman P.C. (1990) Keratin subtypes in carcinomas of the uterin cervix: implications for histiogenesis and differential diagnosis. Cancer Res. 50; 5143-5152. 4. Mommers JM, van Rossum MM, van Erp PE, van De Kerkhof PC. (2000) Changes in keratin 6 and keratin 10 (co-)expression in lesional and symptomless skin of spreading psoriasis. Dermatology 2000;201(1):15-20
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolKRT10, DSEL, TRHDE
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Short nameBioprime
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG1
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SpeciesMouse, Mouses
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Alternative nameCytokeratin 10
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namekeratin 10
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Synonyms gene
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Synonyms gene name
- keratosis palmaris et plantaris
- keratin 10, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-08-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Keratins, type I
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedermatan sulfate epimerase like
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Synonyms gene
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Synonyms gene name
- chromosome 18 open reading frame 4
- dermatan sulfate epimerase-like
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-02-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namethyrotropin releasing hormone degrading enzyme
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Synonyms gene name
- thyrotropin-releasing hormone degrading enzyme
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-05-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M1 metallopeptidases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data