Isolated cytokeratins from human epithelial cells
Cytokeratin 5 and neurofilaments
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
Product is supplied as
Recommended concentration for use
1:10 - 1:50
Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid C or G (Art. No. DE000 and DE007)
Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Smedts F., Ramaekers F., Robben H., et al. (1990) Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J. Pathol. 136,3; 657-668. 3. Eyken P van, et al. (1990) A cytokeratin-immunohistochemical study of hepatoblastoma. Human Pathology 21; 302-308. 4. Broekaert D. Leigh I.M., Laue E.B., Van Muijen G.N.P., Ramackers F.C.S., De Bersaques J. and P. Couke (1993) An immunohistochemical and histochemical study of cytokeratin, involucrin and tranglutaminase in seborrhoeic keratosis. Arch dermatol. Res. 285; 482-490. 5. Verhagen A.P.M. Aalders, T.W., Ramaekers F.C.S., Debruyne F.M.J., and Schalken J.A. (1988) Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration. The Prostate 13; 25-38.