Binding buffer to be used with protein A resins during antibody purification
Protein A IgG Binding Buffer
Applications: Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
BioVision’s ready-to-use Protein A IgG Binding Buffer is optimized to provide the highest IgG binding efficiency when used with Hi-Bind™ Protein A-Agarose (Cat. # 6520), Protein A-Agarose (Cat. # 6526) & Protein A-Sepharose (Cat. # 6501, 6508) or other protein A resins.
Maximum time for storage
Buffering solutions are useful to keep the pH range sable when using this reagent of Biovision.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Protein A IgG Binding
Protein A Immunoglobulin G Binding solution