Mouse anti Cytokeratin 19 / Keratin K19

  • Catalog number
    MUB0329P
  • Price
    Please ask
  • Size
    0,1 mg
  • Category
    Primary Antibodies
  • Long description
    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • Antibody come from
    RCK108 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal preparation of Human bladder carcinoma cell line T24.
  • Other description
    Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
  • Clone
    RCK108
  • Antigen antibody binding interaction
    Mouse anti Cytokeratin 19 / Keratin K19 Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human,Zebrafish
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    RCK108 reacts exclusively with Cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. Does not react with hepatocytes and hepatocellular carcinoma.
  • Research interest
    Cancer,Cytoskeleton
  • Application
    Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
  • Antibody s suited for
    RCK108 is particularly well suited for use in immunohistochemistry on paraffin-embedded tissues after pretreatment with microwave oven based antigen retrieval with destilled water. RCK108 is also useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    Kwaspen, F. H., Smedts, F. M., Broos, A., Bulten, H., Debie, W. M., and Ramaekers, F. C. (1997). Reproducible and highly sensitive detection of the broad spectrum epithelial marker Keratin 19 in routine cancer diagnosis, Histopathology 31, 503-16.
  • Protein number
    P08727
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    KRT19
  • Short name
    Mouse anti Cytokeratin 19 / Keratin K19
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to Cytokeratin 19 / Keratin K19
  • Alternative technique
    murine, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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