GLDH, galactono-1,4 lactone dehydrogenase

  • Catalog number
    AS06 182
  • Price
    Please ask
  • Size
    100 µl
  • Available ordering format
    Lyophilized in PBS pH 7.4
  • Immunogen
    Recombinant C-terminal of Zea mays GLDH, UniProt: C0HFL3
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Total IgG
  • How to reconstitute
    For reconstitution add 100 µl of sterile water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    collection of antibodies to stress proteinscollection of antibodies to mitochondrial proteins
  • Recommended dilutions for use
    1: 5000 with standard ECL (WB)
  • Molecular weight expected аpparent
    68 kDa
  • Verified reactivity
    Avena sativa,Glycine max, Hordeum vulgare, Helianthus annuus, Zea mays
  • Possible reactivity
    Arabidopsis thaliana
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    mitochondrial, membrane or meristematic fractions were shown to be richer in GLDH expression
  • References
    Bartoli et al. (2005). Ascorbate content in wheat leaves is not determined by maximal L-galactono-1, 4-lactone dehydrogenase (GalLDH) activity under drought stress. Plant Cell Environ 28:1073-1081.
  • Scientific context
    Galactono-1,4-lactone dehydrogenase (GLDH) is the enzyme which catalyses last step of ascorbic acid (AA) synthesis in the mitochondria of plant cells. Synonyme: GaLDH
  • Notes
    total IgG concentration is 6.8 ug/ul
  • Protein number
    C0HFL3
  • TAIR number
    Refer to NCBI
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