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Also known as
NOTCH 1
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Other names
neurogenic locus notch homolog protein 1; Neurogenic locus notch homolog protein 1; neurogenic locus notch homolog protein 1; mT14; p300; Motch A; Notch gene homolog 1; major type A protein; transmembrane receptor Notch1; notch 1; Motch A; mT14; p300
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
NOTCH 1
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Gene name synonims
N/A
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Other gene names
Notch1; Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik; Motch; Notch 1; NICD
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
8G10
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Host organism
Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
NOTCH 1 This item specifically recognises Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. _x000D__x000D_The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. _x000D__x000D_Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. _x000D__x000D_Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis._x000D__x000D__x000D_; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
S/N (Tissue Culture Supernatant - liquid)
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Concentration
N/A
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Storage and shipping
Store the antibody should be stored at -20 degrees Celsius. only (ship Store the antibody at +4 degrees Celsius.) Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Flow Cytometry (FACS), Immunohistology - Frozen (IHC), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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French translation
anticorps
