Aggrecanase Assay ELISA (Activity Assay) Kit

  • Catalog number
    30 510 111
  • Price
    Please ask
  • Size
    1 Microplate/ 96 determinations
  • ELISA category
    ELISA (Activity Assay) Kit
  • ELISA detection limits
    0.024 - 1.5 nM ADAMTS4
  • ELISA sensitivity
    12 pM ADAMTS4
  • ELISA s description
    The assay consists of two moduls, the Aggrecanase Modul and the ELISA Module. A recombinant fragment of human aggrecan inter-globular domain (aggrecan-IGD) is first digested with aggrecanase. Proteolytic cleavage of the substrate releases an aggrecan peptide with the N-terminal sequence ARGSVIL (ARGSVIL-peptide). Subsequently, ARGSVIL-neoepitope containing cleavage products are detected and quantified by ELISA using two monoclonal anti-peptide antibodies .
  • Research area interests
    Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
  • Package form
    96 well microplate
  • Antigen
    Aggrecanase
  • Reacts with
    Aggrecanase
  • Tested applications
    The assay measures activity of aggrecanases. It is used to screen and characterize aggrecanase inhibitors.
  • Specificity
    Aggrecanase
  • Crossreactivity
    N/A
  • Preservative materials
    If applicable mentioned in the product data sheet
  • Storage recommendations
    6 month
  • Shipping and storage conditions
    dry Ice and ice pack
  • Long term storage
    -20 °C and 4 °C
  • Assay principle
    Proteolytic Reaction and Sandwich-ELISA
  • Included in the standard package
    microplate, buffers, antibody conjugate,standards
  • Not included in the standard package
    Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
  • Samples to be analyzed
    cell culture
  • PubMed citations
    See the datasheet
  • Technical datasheet
    Contact us to receive the datasheet
  • Representative figures
  • Warnings
    For Research Use only.
  • Test
    biotez supplies other types of Assays as 1. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Gene target
  • Short name
    Aggrecanase Assay ELISA (Activity Assay) Kit
  • Technique
    Assay, ELISA, assays, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
  • Host
    Assay, antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    Aggrecanase test Enzyme-linked immunosorbent assay (Activity test) reagent
  • Alternative technique
    arrays, elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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