RPMI 1640 With L-Glutamine and 25mM HEPES buffer Without L-Arginine and Sodium bicarbonate

  • Catalog number
    AT129-2X10X1L
  • Price
    Please ask
  • Size
    2X10X1L
  • Description
    RPMI 1640, With L-Glutamine and 25mM HEPES buffer Without L-Arginine and Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It was developed for the culture of human normal and neoplastic cells in vitro. It was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines.
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    Off-white to Creamish white, homogenous powder.
  • PH without Sodium Bicarbonate
    6.30 -6.90
  • PH with Sodium Bicarbonate
    6.70 -7.30
  • Osmolality without Sodium Bicarbonate
    280.00 -320.00
  • Osmolality with Sodium Bicarbonate
    320.00 -360.00
  • Cultural Response
    The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content
    NMT 5EU/ml
  • Solubility
    Clear solution at 16.2 gms/L.
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Ordering
    To order RPMI 1640 With L-Glutamine and 25mM HEPES buffer Without L-Arginine and Sodium bicarbonate, please use the Cat. Nr.AT129-2X10X1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for RPMI 1640 With L-Glutamine and 25mM HEPES buffer Without L-Arginine and Sodium bicarbonate
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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