DNA Damage (8-OHdG) ELISA Kit

  • Catalog number
    SKT-120-96S
  • Price
    Please ask
  • Size
    96 well
  • Stock availability
    In Stock
  • Kit s description
    Colorimetric detection of 8-hydroxy-2-deoxy Guanosine
  • Protein target
    DNA Damage (8-OHdG)
  • Scientific context
    8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
  • Primary research area
    Cancer, Oxidative Stress, Cell Signaling, Post-Translational Modifications, Oxidation
  • Category
    Assay ELISA Kits
  • Other name
    8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, 8-OHdG ELISA Kit, DNA Damage ELISA Kit
  • Brand name
    MOLECULAR SIGNATURE®
  • Detection system
    Colorimetric Assay
  • Assay format
    Competitive ELISA (Enzyme-linked Immunosorbent Assay)
  • Main intended usage
    ELISA Kit for 8-OHdG detection in samples.
  • Brief protocol
    1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
  • Kit contents
    96 well microplate for ELISA detection of 8-hydroxy-2-deoxy Guanosine (8-OH-dG)
  • Assay precision
    Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be
  • Assay cross reactivity
    Species Independent
  • Samples to be used with this kit
    Urine, Cell Lysates, Plasma, Sample matrices
  • Maximum samples to be used with this kit
    39 samples in duplicate
  • Assay duration
    1 hour
  • Kit s sensitivity
    0,59 ng/mL
  • Assay detection limit
    0,94 - 60 ng/mL
  • Storage recommendations
    4°C and -20°C
  • Shipping recommendations
    Blue Ice
  • Bibliography
    1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120. 2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34. 3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids. 4. Lin H., et al. (2004) Biochem J. 380: 541-548. 5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666. 6. Lee J., et al. (2005) Hypertension 45: 986-990. 7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152. 8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75. 9. Kuo H., et al. (2007) Mutat Res. 631:62-68. 10. Shen J., et al. (2007) Cancer 109: 574-580. 11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636. 12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110. 13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236. 14. Floyd R.A. (1990) FASEB J 4: 2587-2597.
  • Release date
    1-Aug-2015
  • PubMed number
    27906613, 27821249, 27568862, 27388216, 27084692, 26508318, 26477446, 26635910, 25791998, 25465090, 25524985, 24740568, 24805201, 24215581, 24343823, 24030522, 23993650, 23813449, 23874911, 23510150, 23438443, 24472560, 23069710, 25368634, 22796564, 25538758, 23029301, 21830861, 22790087, 22252725, 22470562, 22036624, 22150331, 22081873, 22041478, 21435260, 21356386
  • Tested applications
    EIA, ELISA
  • Tested reactivity
    Mouse, Human, Mouse, Human, Human, Mouse, Human, Human, Human, Mouse, Zebra finch, Mice, Bird (coal tits), Human, Human, Mouse, Mouse, Human, Mouse, Zebrafish, Human, Mouse, Mouse, Mouse, Mouse, Mouse, Mouse, Zebrafish, Human, Mouse, Human, Mouse, Human, Rat, Human, Human, Mouse
  • Resources available upon request
    Kit Booklet, MSDS, Calculations Worksheet
  • Product image link
  • Product image legend
    Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-120. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.94 - 60 ng/ml. | Chemical Equation of the Oxidation of Guanosine | Diagram of the 8-OHdG Competitive ELISA | Urine Spike Assay | Diagram of the Preparation of the 8-OHdG Standards | Diagram of the Triplicate Sample Plate Format | Preview of the Calculations Worksheet Graph of the Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120 | Chemical Equation of the Oxidation of Guanosine for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120 | Diagram of the 8-OHdG Competitive ELISA for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120 | Graph of the Urine Spike Assay for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120 | Diagram of the Preparation of the 8-OHdG Standards for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120 | Diagram of the Triplicate Sample Plate Format for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120 | Preview of the Calculations Worksheet for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120
  • Warnings
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    2
  • Net weight g
    500
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
    DNA   Damage   8-OHdG  
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    DNA Damage (8-OHdG) ELISA Kit
  • Technique
    ELISA kit, ELISA, dna, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    Desoxyribonucleic acid Damage (8-OHdG) Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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