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Immunogen
Synthetic peptide from the mid-protein of Human Bcl-2
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Antibody s target
Human Bcl-2
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Antibody s full description
Rabbit Anti-Human Bcl-2 Polyclonal
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Primary research fields
Cancer, Apoptosis, Cell Signaling, Metabolism, Mitochondrial Metabolism, Cancer, Invasion/microenvironment, Cancer, Tumor Biomarkers, Oncoproteins, Cancer, Cancer Metabolism, Response to Hypoxia, Cell Signaling, Metabolism, Mitochondrial Metabolism, Mitochondrial Markers
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Protein phosphatase 1 regulatory subunit 50 Antibody, AW986256 Antibody, BCL2_HUMAN Antibody, Bcl2 Antibody, Apoptosis regulator Bcl2 Antibody, BCL2 Antibody, B cell leukemia/lymphoma 2 Antibody, Leukemia/lymphoma, B-cell, 2 Antibody, B cell CLL/lymphoma 2 Antibody, Bcl-2 Antibody, Oncogene B-cell leukemia 2 Antibody, D830018M01Rik Antibody, PPP1R50 Antibody, D630044D05Rik Antibody, Apoptosis regulator Bcl-2 Antibody, C430015F12Rik Antibody
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Verified applications
WB
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Raised in
Rabbit
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Antibody s reactivity
Human, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~26 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-655 was sufficient for detection of Bcl-2 in 15 µg of human 293Trap cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Mitochondrion Outer Membrane, Single-Pass Membrane Protein, Nucleus Membrane, Single-Pass Membrane Protein, Endoplasmic Reticulum Membrane, Single-Pass Membrane Protein
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Tissue specificity
Expressed in a variety of tissues.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
25/Oct/2016
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NCBI number
NP_000624.2
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Gene number
596
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Protein number
P10415
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human 293Trap showing detection of ~26.3 kDa Bcl-2 protein using Rabbit Anti-Bcl-2 Polyclonal Antibody (SPC-655). Lane 1: Molecular Weight Ladder (MW). Lane 2: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody (SPC-655) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~26.3 kDa. Rabbit Anti-Bcl-2 Antibody used in Western blot (WB) on 293Trap (SPC-655)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
