TDGF1, human recombinant

• Catalog number
  4335-1000
• Price
  Please ask
• Size
  1000 ug

• Synonyms
  CRIPTO1, CRIPTO-1, CRGF, Teratocarcinoma-Derived Growth Factor 1, TDGF1, CR1.
• Alternative_names
  CRIPTO1, CRIPTO-1, CRGF, Teratocarcinoma-Derived Growth Factor 1, TDGF1, CR1.
• Description
  A signaling protein involved in embryonic development and tumor growth
• Recombinant
  Yes
• Source
  Human cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Measured by its binding ability in a functional ELISA Immobilized human TDGF1 at 2 ug/ml ( 100 ul/well) can bind human ALK4 with a linear range of 0.0068 - 0.16 ug/ml.
• Molecular Weight
  17.4 kDa
• Storage Temp
  -70°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution Instructions
  Reconstitute in sterile PBS
• Background Information
  Teratocarcinoma-derived growth factor 1, also known as epidermal growth factor-like cripto protein CR1, CRGF, and TDGF1, is a cell membrane which contains one EGF-like domain. In humans, TDGF1 is highly expressed in germ cell tumors and in colon and mammary carcinomas. TDGF1 is a member of the epidermal growth factor-cripto FRL1 cryptic protein family and is involved in the activation of several different signaling pathways during embryonic development and cellular transformation. TDGF1 regulates the activation of several signaling pathways and controls cellular transformation in embryonic status. Patients with high TDGF1 expression were statistically susceptible to a recurrence of the disease, and showed poorer disease-free survival than those with low expression. TDGF1 is a predictive marker for metachronous metastasis in colorectal cancer (CRC) patients. It is preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. TDGF1 plays a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
• Amino acid sequence
  A DNA sequence encoding the human TDGF1 (AAH22393.1) (Met 1 - Thr 172) with a C-terminal polyhistidine tag was expressed.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TDGF1  
• Gene symbol
  TDGF1
• Short name
  TDGF1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  teratocarcinoma-derived growth factor 1, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  teratocarcinoma-derived growth factor 1, CR and CRGF and CRIPTO, TDGF1 and IDBG-409017 and ENSG00000241186 and 6997, growth factor activity, nuclei, Tdgf1 and IDBG-202568 and ENSMUSG00000032494 and 21667

Gene info

• Identity
  HGNC:11701
• Gene
  TDGF1
• Long gene name
  teratocarcinoma-derived growth factor 1
• Synonyms
  • CRIPTO
  • CR
  • Cripto-1
  • CR-1
• GenBank acession
  • M96955
• Locus
  3p21.31
• Discovery year
  1990-08-02
• Entrez gene record
  6997
• Pubmed identfication
  • 1882841
  • 10393436
• RefSeq identity
  • NM_003212
• VEGA ID
  OTTHUMG00000191980

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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