MDA-MB-231 Cell Line

• Catalog number
  EP-CL-0150
• Price
  Please ask
• Size
  1×10⁶ cells/vial

• Abbreviation
  MDA-MB-231
• Leadtime
  3-5 days
• Shipping condition
  Dry ice
• Storage
  Liquid nitrogen -196°C
• Age
  51 years adult
• Morphology
  Epithelial
• Growth_properties
  Adherent
• Doubling_time
  NA
• Background
  NA
• Biosafety
  1
• Medium
  DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
• Subculturing
  Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
• Ratio
  1:2-1:4
• Renewal
  Every 2 to 3 days
• Cryopreservation
  Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
• Culture_conditions
  Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
• Tumorigenic
  Yes
• Effects
  Yes, in ALS treated BALB/c mice, forms poorly differentiated adenocarcinoma(gradeⅢ). Yes, in nude mice, forms poorly differentiated adenocarcinoma(gradeⅢ).
• Receptor_expression
  Epidermal growth factor(EGF), expressed; transforming growth factor alpha(TGF alpha), expressed
• Antigen_expression
  NA
• Gene_expression
  NA
• Applications
  Transfection.
• Duration
  NA
• Technical data
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  MDA-MB-231   Line  
• Gene symbol
  MB, CD79A, SDCBP, IFIH1, IL24
• Short name
  MDA-MB-231 Line
• Species
  Homo sapiens, Human
• Alternative name
  MDA-myoglobin-231 cellular Line
• Alternative to gene target
  myoglobin, myoglobgin and PVALB, MB and IDBG-5491 and ENSG00000198125 and 4151, heme binding, multiple, Mb and IDBG-157509 and ENSMUSG00000018893 and 17189, MB and IDBG-637940 and ENSBTAG00000005333 and 280695
• Tissue
  Mammary gland/breast, derived from metastatic site: pleural effusion

Gene info

• Identity
  HGNC:6915
• Gene
  MB
• Long gene name
  myoglobin
• Synonyms
  • PVALB
• Locus
  22q12.3
• Discovery year
  2001-06-22
• Entrez gene record
  4151
• Pubmed identfication
  • 10591208
  • 2989088
• RefSeq identity
  • NM_203377
• VEGA ID
  OTTHUMG00000150606

Gene info

• Identity
  HGNC:1698
• Gene
  CD79A
• Long gene name
  CD79a molecule
• Synonyms gene
  • IGA
• Synonyms gene name
  • CD79A antigen (immunoglobulin-associated alpha)
  • CD79a molecule, immunoglobulin-associated alpha
• Synonyms
  • MB-1
• Synonyms name
  • B-cell antigen receptor complex-associated protein alpha chain
• GenBank acession
  • M80462
• Locus
  19q13.2
• Discovery year
  1992-07-31
• Entrez gene record
  973
• Pubmed identfication
  • 1538135
• Classification
  • CD molecules
  • V-set domain containing
• VEGA ID
  OTTHUMG00000182678
• Locus Specific Databases
  • CD79Abase: Mutation registry for Igα deficiency
  • LRG_42

Gene info

• Identity
  HGNC:10662
• Gene
  SDCBP
• Long gene name
  syndecan binding protein
• Synonyms
  • SYCL
  • MDA-9
• Synonyms name
  • syntenin
  • syntenin-1
• GenBank acession
  • AF000652
• Locus
  8q12.1
• Discovery year
  1998-02-11
• Entrez gene record
  6386
• Pubmed identfication
  • 9391086
• RefSeq identity
  • NM_005625
• Classification
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000164303

Gene info

• Identity
  HGNC:18873
• Gene
  IFIH1
• Long gene name
  interferon induced with helicase C domain 1
• Synonyms
  • MDA-5
  • Hlcd
  • MDA5
  • IDDM19
• Synonyms name
  • helicard
  • melanoma differentiation-associated gene 5
• GenBank acession
  • AF095844
• Locus
  2q24.2
• Discovery year
  2004-06-25
• Entrez gene record
  64135
• RefSeq identity
  • NM_022168
• Classification
  • RNA helicases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000132055
• Locus Specific Databases
  • LRG_1235

Gene info

• Identity
  HGNC:11346
• Gene
  IL24
• Long gene name
  interleukin 24
• Synonyms gene
  • ST16
• Synonyms
  • mda-7
  • IL10B
  • Mob-5
  • C49A
  • FISP
  • IL-24
• Synonyms name
  • melanoma differentiation association protein 7
  • suppression of tumorigenicity 16 (melanoma differentiation)
  • IL-4-induced secreted protein
• GenBank acession
  • U16261
• Locus
  1q32.1
• Discovery year
  1999-12-02
• Entrez gene record
  11009
• Pubmed identfication
  • 8545104
  • 8799171
• RefSeq identity
  • NM_006850
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000036459

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