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Description
Tryptose, Certified is used in the culture media wth requirement of fastidious growth of microorganisms, used also in the production of vaccins and tissuecultue media. It is a combinated enzymatically digested protein with one nutritional properties
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Appearance
Light yellow to brownish yellow homogenous free flowing powder,having characteristic odour but not putrescent.
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Solubility
Freely soluble in distilled/purified water, insoluble in alcohol.
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Clarity
1% w/v aqueous solution remains clear without haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes
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Reaction
Reaction of 2% w/v aqueous solution at 25ºC.
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PH
6.20- 7.20
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Total aerobic microbial count cfu gm
By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count :
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Total Yeast and mould count cfu gm
By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count :
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Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosaNegative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample
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Indole Test
Tryptophan content: Passes
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Cultural response
Cultural response observed after an incubation at 35-37ºC for 18-24 hours by preparing Lauryl Tryptose Broth (M080) using Tryptose as an ingredient.
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Chemical Analysis
TotalNitrogen >= 12.0% AminoNitrogen >= 3.0% Sodium chloride
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Storage
Store below 30°C.
