Nicotinamide N-Methyltransferase, Human Recombinant (hNNMT)
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Catalog number
7261-1000
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Price
Please ask
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Size
1 mg
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Synonyms
NNMT
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Alternative_names
NNMT
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Description
A cytosolic methyltransferase catalyzing the N-methylation of nicotinamide
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Molecular Weight
31.7 kDa (263aa + NT 6xHis-tag)
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Storage Temp
-80°C
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Shipping
Dry Ice
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Shelf Life
12 months
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Concentration
3.42 mg/ml
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Appearance
Liquid
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Physical form description
3.42 mg/ml solution in 25 mM Tris (pH8.0) buffer containing 20% glycerol
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Background Information
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is an important cytosolic methyltransferase catalyzing the N-methylation of nicotinamide, pyridines and structural analogs, playing a crucial role in the biotransformation and detoxification of many xenobiotic compounds. In the N-methylation process, NNMT uses S-adenosyl methionine as the methyl donor and nicotinamide as methyl acceptor. NNMT is mostly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. It may also play an important role in regulating biological processes related to N-methyl nicotinamide such as anti-inflammatory, anti-thrombotic, vasoprotective, and gastroprotective properties. Increased NNMT activity was reported in many kinds of tumors. NNMT is a potential biomarker and therapeutic target in caner diagnosis and treatment. NNMT serum levels have significance in the premature detection and in the management of patients with colorectal cancer. The recombinant protein includes hNNMT with N-terminal His-tag.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Nicotinamide N-Methyltransferase, Recombinant (hNNMT)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Nicotinamide N-Methyltransferase, H. sapiens Rec. (hNNMT)
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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