miR Multi Assay kit System I Real-Time PCR Master mix kit
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Catalog number
HMI-S102-500
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Price
Please ask
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Size
500 reactions
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Product configuration
One box of the product miR Multi Assay kit System I Real-Time PCR Master mix kit includes tubes labelled as 4. I Oligo5. I 2X SYBR Green Master mix
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Short description
This kit represents a set of the following reagents: Reverse Transcriptase, Poly A polymerase
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Type of the probes
SYBR green labeled probes
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Storage conditions
Upon receipt, store the product miR Multi Assay kit System I Real-Time PCR Master mix kit according to the instructions described in the manual to keep the quality and stability of the reagents.
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Tips
This product is manufactured for research purposes only and it is not tested for diagnostics applications.
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Test
Heimbiotek supplies other types of Assays as 1.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
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Description
Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
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Gene target
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Short name
Real-Time PCR Master mix kit
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Technique
Real-time, Assay, multi, PCR, assays, multi, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Host
Assay
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Alternative name
miR Multi test reagent System I Real-Time PCR test kit Master mix reagent
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Alternative technique
rtpcr, arrays, kits, dna-amplification
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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