One box of the product miR Multi Assay kit System I Real-Time PCR Master mix kit includes tubes labelled as 4. I Oligo5. I 2X SYBR Green Master mix
This kit represents a set of the following reagents: I 10X RT buffer
Type of the probes
SYBR green labeled probes
Upon receipt, store the product miR Multi Assay kit System I Real-Time PCR Master mix kit according to the instructions described in the manual to keep the quality and stability of the reagents.
Heimbiotek supplies other types of Assays as 1.
PCR, polymerase chain reaction
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.
Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
miR Multi Assay kit System I Real-Time PCR Master kit
Real-time, Assay, multi, PCR
miR Multi test reagent System I Real-Time PCR test reagent Master mix reagent