Recombinant Rat CD6 / TP120 Protein (His tag)

• Catalog number
  PKSR030241-100µg
• Price
  Please ask
• Size
  100µg

• Synonym
  CD6
• Activity
  1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3 , The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ratCD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37℃.
• Sequence
  Met1-Asn396
• Fusion tag
  C-His
• Accession
  Q812A4
• ExpressedHost
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  42.5 kDa
• AP Mol Mass
  64-70 KDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
• Technical data
• Conjugation
  histidine
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD6   TP120   Protein   His   tag  
• Gene symbol
  CD6
• Short name
  Recombinant CD6 / TP120 Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  His
• Species
  Rat, Rats
• Alternative name
  Rec. Rat CD6 molecule / TP120 Protein (histidine detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  CD6 molecule, TP120, CD6 and IDBG-49617 and ENSG00000013725 and 923, protein binding, Plasma membranes, Cd6 and IDBG-145044 and ENSMUSG00000024670 and 12511, CD6 and IDBG-643095 and ENSBTAG00000018367 and 282881

Gene info

• Identity
  HGNC:1691
• Gene
  CD6
• Long gene name
  CD6 molecule
• Synonyms gene name
  • CD6 antigen
• Synonyms
  • Tp120
• Locus
  11q12.2
• Discovery year
  1997-07-04
• Entrez gene record
  923
• Pubmed identfication
  • 9013954
• RefSeq identity
  • NM_006725
• Classification
  • Scavenger receptor cysteine rich domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000167823

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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