Nipah Virus (NiV) Real Time RT-PCR Reagent I and II

  • Catalog number
    NIV ER-0170-01
  • Price
    Please ask
  • Size
    25tests / kit
  • Description
    Real time PCR assay for detecting the Nipah virus. It is ready to use kit. It contains negative control, positive control, master mix, primer and probes. This assay can be used immediately after the arrival of kit. It is highly specific kit for Nipah virus.
  • Application
    Diagostic of Nipah Virus contamination
  • Intended Use
    detection of Nipah Virus in serum, plasma, infected animal tissue or secretion by RT PCR systems.
  • Diseases
    encephalitis, respiratory diseases.
  • Cotamiation
    Bat - Fruits and vegetables
  • Nucleic acid
    RNA
  • Kit Contents
    NiV Super Mix 1 vial, 480µl RT-PCR Enzyme Mix 1 vial, 28µl Molecular Grade Water 1 vial, 400μl Internal Control 1 vial, 30μl NiV Positive Control 1 vial, 30μl
  • Storage
    All reagents should be stored at -20°C. Storage at +4°C is not recommended.
  • Shelf life
    All reagents can be used until the expiration date indicated on the kit label
  • Precautions
    Repeated thawing and freezing (> 3x) should be avoided, as this may reduce the sensitivity of th assay. Cool all reagents during the working steps. Super Mix should be stored in the dark.
  • Instrumets
    LightCycler 1.0 (Internal control can't be used for this system) and LightCycler 2.0
  • Outbreak
    India - Bagladech - Kuwait
  • Procedure
    1.RNA-Extraction, 2.Internal Control , 3.RT-PCR Protocol
  • Note
    For in vitro diagnostic use only.
  • CE
    CE marked
  • Group
    PCR, polymerase chain reaction, RT-PCR mixes
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene target
    Nipah   Virus   NiV   Real   Time   Reagent  
  • Short name
    Nipah Virus (NiV) Real Time RT-PCR Reagent I II
  • Technique
    RT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Species
    Virus, Viruses
  • Alternative name
    Nipah Virus (NiV) Real Time Reverse transcription PCR test kit Reagent I and II
  • Alternative technique
    rtpcrkits, dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
  • Tree numbers
    • E05.393.620
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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