Nipah Virus (NiV) Real Time RT-PCR Reagent I and II
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Catalog numberNIV ER-0170-01
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PricePlease ask
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Size25tests / kit
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DescriptionReal time PCR assay for detecting the Nipah virus. It is ready to use kit. It contains negative control, positive control, master mix, primer and probes. This assay can be used immediately after the arrival of kit. It is highly specific kit for Nipah virus.
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ApplicationDiagostic of Nipah Virus contamination
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Intended Usedetection of Nipah Virus in serum, plasma, infected animal tissue or secretion by RT PCR systems.
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Diseasesencephalitis, respiratory diseases.
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CotamiationBat - Fruits and vegetables
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Nucleic acidRNA
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Kit ContentsNiV Super Mix 1 vial, 480µl RT-PCR Enzyme Mix 1 vial, 28µl Molecular Grade Water 1 vial, 400μl Internal Control 1 vial, 30μl NiV Positive Control 1 vial, 30μl
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StorageAll reagents should be stored at -20°C. Storage at +4°C is not recommended.
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Shelf lifeAll reagents can be used until the expiration date indicated on the kit label
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PrecautionsRepeated thawing and freezing (> 3x) should be avoided, as this may reduce the sensitivity of th assay. Cool all reagents during the working steps. Super Mix should be stored in the dark.
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InstrumetsLightCycler 1.0 (Internal control can't be used for this system) and LightCycler 2.0
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OutbreakIndia - Bagladech - Kuwait
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Procedure1.RNA-Extraction, 2.Internal Control , 3.RT-PCR Protocol
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NoteFor in vitro diagnostic use only.
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CECE marked
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GroupPCR, polymerase chain reaction, RT-PCR mixes
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short nameNipah Virus (NiV) Real Time RT-PCR Reagent I II
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TechniqueRT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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SpeciesVirus, Viruses
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Alternative nameNipah Virus (NiV) Real Time Reverse transcription PCR test kit Reagent I and II
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Alternative techniquertpcrkits, dna-amplification
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MeSH Data
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Name
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ConceptScope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
- E05.393.620
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data