CE
CE marked
Description
Real time PCR assay for detecting the Nipah virus. It is ready to use kit. It contains negative control, positive control, master mix, primer and probes. This assay can be used immediately after the arrival of kit. It is highly specific kit for Nipah virus.
Cotamiation
Bat - Fruits and vegetables
Shelf life
All reagents can be used until the expiration date indicated on the kit label
Diseases
encephalitis, respiratory diseases.
Kit Contents
NiV Super Mix 1 vial, 480µl RT-PCR Enzyme Mix 1 vial, 28µl Molecular Grade Water 1 vial, 400μl Internal Control 1 vial, 30μl NiV Positive Control 1 vial, 30μl
Storage
All reagents should be stored at -20°C. Storage at +4°C is not recommended.
Outbreak
India - Bagladech - Kuwait
Note
For in vitro diagnostic use only.
Procedure
1.RNA-Extraction, 2.Internal Control , 3.RT-PCR Protocol
Intended Use
detection of Nipah Virus in serum, plasma, infected animal tissue or secretion by RT PCR systems.
Precautions
Repeated thawing and freezing (> 3x) should be avoided, as this may reduce the sensitivity of th assay. Cool all reagents during the working steps. Super Mix should be stored in the dark.
Application
Diagostic of Nipah Virus contamination
Nucleic acid
RNA
Group
PCR, polymerase chain reaction,RT-PCR mixes
About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
