Nipah Virus (NiV) Real Time RT-PCR Reagent III and IV
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Catalog numberNIV ER-0170-02
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PricePlease ask
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Size25tests / kit
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DescriptionReal time PCR assay for detecting the Nipah virus. It is ready to use kit. It contains negative control, positive control, master mix, primer and probes. This assay can be used immediately after the arrival of kit. It is highly specific kit for Nipah virus.
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ApplicationDiagostic of Nipah Virus contamination
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Intended Usedetection of Nipah Virus in serum, plasma, infected animal tissue or secretion by RT PCR systems.
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Diseasesencephalitis, respiratory diseases.
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CotamiationBat - Fruits and vegetables
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Nucleic acidRNA
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Kit ContentsNiV Super Mix 1 vial, 480µl RT-PCR Enzyme Mix 1 vial, 28µl Molecular Grade Water 1 vial, 400μl Internal Control 1 vial, 30μl NiV Positive Control 1 vial, 30μl
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StorageAll reagents should be stored at -20°C. Storage at +4°C is not recommended.
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Shelf lifeAll reagents can be used until the expiration date indicated on the kit label
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PrecautionsRepeated thawing and freezing (> 3x) should be avoided, as this may reduce the sensitivity of th assay. Cool all reagents during the working steps. Super Mix should be stored in the dark.
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InstrumetsPE5700, MJ-Opticon & other single color systems and ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems
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OutbreakIndia - Bagladech - Kuwait
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Procedure1.RNA-Extraction, 2.Internal Control , 3.RT-PCR Protocol
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NoteFor in vitro diagnostic use only.
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CECE marked
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GroupPCR, polymerase chain reaction, RT-PCR mixes
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbolCPLX3, MGAT3, MGST3, P4HA3, CASP5, IGHVIII-82, THAP4, MT-CSB3, IGHVIII-44, IGHVIII-67-4
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Short nameNipah Virus (NiV) Real Time RT-PCR Reagent III IV
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TechniqueRT-PCR, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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SpeciesVirus, Viruses
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Alternative nameNipah Virus (NiV) Real Time Reverse transcription PCR test kit Reagent III and IV
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Alternative techniquertpcrkits, dna-amplification
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Gene info
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Identity
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Gene
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Long gene namecomplexin 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
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Synonyms gene name
- mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit alpha 3
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
- prolyl 4-hydroxylase, alpha polypeptide III
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 5
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Synonyms gene name
- caspase 5, apoptosis-related cysteine protease
- caspase 5, apoptosis-related cysteine peptidase
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Synonyms
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Locus
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Discovery year1996-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-82 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-82
- immunoglobulin heavy variable (III)-82 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrially encoded conserved sequence block III
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Synonyms gene
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Synonyms gene name
- conserved sequence block III
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Synonyms
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Locus
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Discovery year1989-10-11
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Pubmed identfication
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Classification
- Mitochondrially encoded regions
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-44 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-44
- immunoglobulin heavy variable (III)-44 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-67-4 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-67-4
- immunoglobulin heavy variable (III)-67-4 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
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Tree numbers
- E05.393.620.500.706
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data