Rabbit Anti-Human GIT1 (pTyr545) Polyclonal: RPE

• Catalog number
  SPC-987D-RPE
• Price
  Please ask
• Size
  100 µl

• Immunogen
  A phospho-specific peptide corresponding to residues surrounding Tyr545 of human GIT1 (AA542-548)
• Antibody s target
  Human GIT1 (pTyr545)
• Antibody s full description
  Rabbit Anti-Human GIT1 (pTyr545) Polyclonal
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  ARF GAP GIT1 Antibody, ARF GTPase activating protein GIT1 Antibody, GIT1 Antibody, Cool associated and tyrosine phosphorylated protein 1 Antibody, G protein coupled receptor kinase interactor 1 Antibody, GRK interacting protein 1 Antibody
• Verified applications
  WB, AM
• Raised in
  Rabbit
• Antibody s reactivity
  Human
• Antibody s recommended dilutions for use
  WB (1:250); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS pH7.4, 50% glycerol, 0.025% Thimerosal
• Antibody s concentration
  See included datasheet or contact our support service
• Antibody s specificity
  Detects 84.341 kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:250 dilution of SPC-987 was sufficient for detection of GIT1 (pTyr545) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody in cell
  Aggresome, Cytoplasm, Cytosol, Focal Adhesion, Membrane
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.
• Bibliography
  Refer to PubMed
• Released date
  29/Aug/2016
• NCBI number
  NP_001078923
• Gene number
  28964
• Protein number
  Q9Y2X7
• PubMed number
  Refer to PubMed
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  No Data Available
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Test
  StressMark antibodies supplies antibodies that are for research of human proteins.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  GIT1   pTyr545   RPE  
• Gene symbol
  RPE, GIT1
• Short name
  Rabbit Anti- GIT1 (pTyr545) Polyclonal: RPE
• Technique
  anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  RPE
• Species
  Human, Humans
• Alternative name
  production species: rabbit Antibody toHuman GIT1 (pTyr545) polyclonal: RPE
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:10293
• Gene
  RPE
• Long gene name
  ribulose-5-phosphate-3-epimerase
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  6120
• RefSeq identity
  • NM_006916
• VEGA ID
  OTTHUMG00000154690

Gene info

• Identity
  HGNC:4272
• Gene
  GIT1
• Long gene name
  GIT ArfGAP 1
• Synonyms gene name
  • G protein-coupled receptor kinase interactor 1
  • G protein-coupled receptor kinase interacting ArfGAP 1
• GenBank acession
  • AF124490
• Locus
  17q11.2
• Discovery year
  2000-07-31
• Entrez gene record
  28964
• Pubmed identfication
  • 9826657
  • 10896954
• RefSeq identity
  • NM_014030
• Classification
  • ArfGAPs
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000132730

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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