-
Type
Conjugated Primary Antibody
-
Conjugated with
ALEXA FLUOR® 594
-
Host organism
Rabbit (Oryctolagus cuniculus)
-
Target Protein Peptide
p73 Tyr99
-
Specificity
This antibody reacts specifically with p73(Tyr99)
-
Modification
Phosphorylation
-
Modification site
Tyr99
-
Clonality
Polyclonal Antibody
-
Clone
Polyclonal Antibodies
-
Concentration
1ug per 1ul
-
Subcellular locations
N/A
-
Antigen Source
KLH conjugated synthetic phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 [SP(p-Y)AQ]
-
Gene ID
7161
-
Swiss Prot
N/A
-
Applications
IF(IHC-P)
-
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
-
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background information
P73 protein is a structural and functional homologue of p53, a tumor suppressor gene. In this study, The p73 protein, p19ras, by the yeast two-hybrid screening method. Alternative splicing of the proto-oncogene H-ras pre-mRNA has led to two distinct transcripts, Ras proteins are known to be small membrane-localized guanine nucleotide-binding proteins. However, unlike other Ras proteins, p19ras is localized in the nucleus and the cytosol and its interaction with P73 protein occurred exclusively in the nucleus. Oncogenic MDM2 (mouse double minutes 2) is a known repressor of p73 transcriptional activity. In this study, when p19ras was bound to MDM2, it further inhibited the association of MDM2 to the p73 protein. Therefore, this study presents a novel pathway of Ras signaling that occurs in the nucleus, involving p19ras and p73.
-
Purification method
Purified by Protein A.
-
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission
590nm/617nm
-
Synonyms
p73Tyr99; TP73phospho Y99; p53 like transcription factor; p53 Related Protein; p73; TP 73; TP73; Tumor Protein p73; P73_HUMAN.
-
Also known as
p73(Tyr99) Polyclonal Antibody
-
Other name
Anti-p73(Tyr99) Polyclonal
-
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Properties
For facs or microscopy Alexa 1 conjugate.
-
Conjugation
Alexa Fluor, ALEXA FLUOR® 594
-
Conjugated
Alexa conjugate 1
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.