WNT-1, human recombinant
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Catalog number4754-50
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PricePlease ask
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Size50 ug
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SynonymsINT-1
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Alternative_namesINT-1, Proto-oncogene Int-1 homolog
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DescriptionA proto-oncogene implicated in oncogenesis and in several developmental processes
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityThe ED₅₀ was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED₅₀ for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
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Results1.5 - 2.5 ng/m
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Molecular Weight38.4 kDa
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Storage Temp-20°C
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ShippingBlue ice
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionSterile filtered and lyophilized with no additives
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Reconstitution InstructionsReconstitute in H₂O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers
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Background InformationWnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intraCellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolWNT1, WNT7A, WNT4, WNT10B, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1
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Short nameWNT-1, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameWNT-1, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameWnt family member 1
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Synonyms gene
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Synonyms gene name
- wingless-type MMTV integration site family, member 1
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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Classification
- Wnt family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWnt family member 7A
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Synonyms gene name
- wingless-type MMTV integration site family, member 7A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-03-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Wnt family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWnt family member 4
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Synonyms gene name
- wingless-type MMTV integration site family, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-07-31
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Entrez gene record
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Pubmed identfication
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Classification
- Wnt family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWnt family member 10B
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Synonyms gene name
- wingless-type MMTV integration site family, member 10B
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-09-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Wnt family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data