Mouse anti OB-Cadherin / Cadherin-11

• Catalog number
  MUB0306P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the Catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migRation of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
• Antibody come from
  16A is a Mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with affinity purified extracellular domain of Human cadherin-11-GST fusion protein.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  16A
• Antigen antibody binding interaction
  Mouse anti OB-Cadherin / Cadherin-11 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human,Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  16A recognizes the extracellular domain of cadherin-11.
• Research interest
  Cell adhesion
• Application
  Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
• Antibody s suited for
  16A is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 – 1:250 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Bussemakers, M. J., Van Bokhoven, A., Tomita, K., Jansen, C. F., and Schalken, J. A. (2000). Complex cadherin expression in Human prostate cancer cells, Int J Cancer 85, 446-50. Tomita, K., van Bokhoven, A., van Leenders, G. J., Ruijter, E. T., Jansen, C. F., Bussemakers, M. J., and Schalken, J. A. (2000). Cadherin switching in Human prostate cancer progression, Cancer Res 60, 3650-4.
• Protein number
  P55287
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  OB-Cadherin   Cadherin-11  
• Gene symbol
  CDH11, LEP, LEPROT, SIGLEC5, SIGLEC6
• Short name
  Mouse anti OB-Cadherin / Cadherin-11
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to OB-Cadherin / Cadherin-11
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:1750
• Gene
  CDH11
• Long gene name
  cadherin 11
• Synonyms gene name
  • cadherin 11, type 2, OB-cadherin (osteoblast)
• Synonyms
  • OB
  • CAD11
• Synonyms name
  • OB-Cadherin
• GenBank acession
  • D21255
• Locus
  16q21
• Discovery year
  1995-03-30
• Entrez gene record
  1009
• Pubmed identfication
  • 9615235
• RefSeq identity
  • NM_033664
• Classification
  • Type II classical cadherins
• VEGA ID
  OTTHUMG00000137494

Gene info

• Identity
  HGNC:6553
• Gene
  LEP
• Long gene name
  leptin
• Synonyms gene
  • OBS
  • OB
• Synonyms gene name
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
• Locus
  7q32.1
• Discovery year
  1993-01-26
• Entrez gene record
  3952
• Pubmed identfication
  • 1686014
  • 16932309
• Classification
  • Neuropeptides
• VEGA ID
  OTTHUMG00000157564

Gene info

• Identity
  HGNC:29477
• Gene
  LEPROT
• Long gene name
  leptin receptor overlapping transcript
• Synonyms
  • OBRGRP
  • OB-RGRP
  • VPS55
  • FLJ90360
• Synonyms name
  • leptin receptor gene related protein
  • endospanin-1
  • endospanin
• GenBank acession
  • Y12670
• Locus
  1p31.3
• Discovery year
  2004-11-16
• Entrez gene record
  54741
• Pubmed identfication
  • 9207021
  • 21454707
• RefSeq identity
  • NM_017526
• VEGA ID
  OTTHUMG00000009065

Gene info

• Identity
  HGNC:10874
• Gene
  SIGLEC5
• Long gene name
  sialic acid binding Ig like lectin 5
• Synonyms gene
  • CD33L2
• Synonyms gene name
  • sialic acid binding Ig-like lectin 5
• Synonyms
  • OB-BP2
  • SIGLEC-5
  • CD170
• GenBank acession
  • U71383
• Locus
  19q13.41
• Discovery year
  1998-12-16
• Entrez gene record
  8778
• Pubmed identfication
  • 10343116
• RefSeq identity
  • NM_003830
• Classification
  • CD molecules
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000165510

Gene info

• Identity
  HGNC:10875
• Gene
  SIGLEC6
• Long gene name
  sialic acid binding Ig like lectin 6
• Synonyms gene
  • CD33L
  • CD33L1
• Synonyms gene name
  • sialic acid binding Ig-like lectin 6
• Synonyms
  • OB-BP1
  • SIGLEC-6
  • CD327
• GenBank acession
  • D86358
• Locus
  19q13.41
• Discovery year
  1996-12-17
• Entrez gene record
  946
• Pubmed identfication
  • 9465907
• RefSeq identity
  • NM_001245
• Classification
  • I-set domain containing
  • Sialic acid binding Ig like lectins
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000133571

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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