CDC25A Antibody (phospho-S124)

• Catalog number
  F48374-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CDC25A (phospho-S124)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
• Notes
  Titration of the phospho-CDC25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This phospho-CDC25A antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P30304
• Purity
  Antigen affinity
• Description
  CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
• Immunogen
  This phospho-CDC25A antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS124 of human CDC25A.
• Storage
  Aliquot the phospho-CDC25A antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CDC25A   phospho-S124  
• Gene symbol
  CDC25A
• Short name
  Anti-CDC25A (phospho-S124)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to CDC25A (phospho-S124)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1725
• Gene
  CDC25A
• Long gene name
  cell division cycle 25A
• Synonyms name
  • CDC25 homolog A (S. pombe)
• GenBank acession
  • M81933
• Locus
  3p21.31
• Discovery year
  1992-07-31
• Entrez gene record
  993
• Pubmed identfication
  • 1836978
• RefSeq identity
  • NM_001789
• Classification
  • Class III Cys-based CDC25 phosphatases
• VEGA ID
  OTTHUMG00000133535

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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