Ovine Clusterin(CLU) ELISA kit
ELISA test kit for Sheep Clusterin(CLU)
Summary of the prodecure
1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
ELISA test kit for Sheep Clusterin(CLU)Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
Shipping, storage, shelf life
The Clusterin(CLU) ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Sheep Clusterin(CLU) ELISA kit is 6 months after delivery.
Our Clusterin(CLU) ELISA kit has been proven to work and successfully detect Clusterin(CLU) in Ovine
Enzyme Linked Immunosorbent Assay
This is a ready to use kit for performing ELISA tests for detection of Clusterin(CLU) in samples from Sheep
Due to transportation and handling small amounts of the liquid components of the Ovine Clusterin(CLU) ELISA kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Clusterin(CLU) ELISA kit
ELISA kit, ELISA
ovine Clusterin(clusterin) Enzyme-linked immunosorbent assay reagent
Alternative to gene target
clusterin, APO-J and APOJ and CLI and CLU1 and CLU2 and KUB1 and NA1/NA2 and SGP-2 and SGP2 and SP-40 and TRPM-2 and TRPM2, CLU and IDBG-14287 and ENSG00000120885 and 1191, misfolded protein binding, nuclei, Clu and IDBG-174736 and ENSMUSG00000022037 and 12759, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832