0.5mg/ml if reconstituted with 0.2ml sterile DI water
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
WB, IHC-P, ICC
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
The stated application concentrations are suggested starting amounts. Titration of the XRCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
X-ray repair, complementing defective, in chinese hamster 1 is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The protein interacts with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.
An amino acid sequence from the N-terminus of human XRCC1 (QDSTHCAENLLKADTYRKWR) was used as the immunogen for this XRCC1 antibody (100% mouse homology).
After reconstitution, the XRCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Long gene name X-ray repair cross complementing 1
Synonyms gene name
- X-ray repair complementing defective repair in Chinese hamster cells 1
Discovery year 2001-06-22
Entrez gene record 7515
Havana BLAST/BLAT OTTHUMG00000182699