Scl-70, Recombinant, Human, Western Blot Positive Control (Scleroderma, DNA Topoisomerase I)
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Catalog number
MBS637451
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Price
Please ask
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Size
0.1 mL
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Products_type
Recombinant Protein
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Products_short_name
[Scl-70, Western Blot Positive Control]
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Purity
PurifiedPurified
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Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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Storage_stability
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
Western Blot (WB)
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
positif, recombinants
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Source
Recombinants or rec. proteins
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Scl-70, Recombinant, , Blot Positive Control (Scleroderma, DNA Topoisomerase I)
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Technique
Western Blot, Recombinant, Positive, Control, Blot, dna, western Blots, controls, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Scl-70, Rec., H. sapiens, Western Blot Positive reference (Scleroderma, Desoxyribonucleic acid Topoisomerase I)
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Alternative technique
rec, controls, western
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Tissue
control
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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